E. coli NEc1


E. coli strain derived from BL21(DE3) and used for minimizing SlyD contamination in 6-His tagged protein preparations. Reduced contamination of recombinant His-tagged protein or polyhistidine-tagged protein purified by immobilized metal affinity chromatography (IMAC)

Description
SpeciesEscherichia coli
Name

NEC1
Parent
E. coli BL21(DE3)
Genotype

fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5 slyD∆(His151_His196)
Construction
Nucleotides 450-588 of slyD gene were deleted by homologous recombination. This slyD mutation is the same as in the strain LOBSTR (Andersen 2013).
Reference(s)
Reference Title PMID/ISBN
Andersen et al. 2013 Optimized E. coli expression strain LOBSTR eliminates common contaminants from His-tag purification 23852738