Solutions

Name	Use	Description
TNE, 10X	Component of DNA/RNA concentration assay with ethidium bromide	Tris-Cl - EDTA - NaCl
TNT		Tris NaCl TWEEN 20 Buffer pH 8.0
Towbin Buffer		Towbin buffer
Towbin Buffer with SDS		Towbin Transfer Buffer with SDS
TPE, 10X	Electrophoresis running buffer	Tris - Phosporic Acid - EDTA
Transcription Buffer, 10X	Nuclease S1 RNA protection	Tris-Cl pH 7.5 - NaCl - MgCl2 - Spermidine
Transfer Buffer - Alkaline Transfer of Nucleic Acids to Charged Membranes	Hybridization analysis of nucleic acids	NaOH - NaCl
Transfer Buffer 1 (For Proteins 20-400 kD)	Western blotting	Tris - Glycine - SDS - Methanol
Transfer Buffer 2 (For Proteins <80 kD)	Western blotting	Tris - Glycine - SDS - Methanol
Tris Protein Extraction Buffer I, 10X	Protein extraction (Buffer H)	Tris-Cl pH 8.0 - EDTA
Tris-Cl pH 7.1, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 7.2, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 7.3, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 7.4, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 7.5, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 7.6, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 7.7, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 7.8, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 7.9, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 8.0, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 8.1, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 8.2, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 8.3, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 8.4, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 8.5, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 8.6, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 8.7, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 8.8, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Cl pH 8.9, 50 mM	Tris Buffer	Tris Base - HCl
Tris-Formamide Hybridization Buffer	Southern blotting	Tris-Cl pH 7.5 - NaCl - EDTA - Formamide
Tris-Glycine SDS Electrophoresis Buffer, 10X		Tris-Glycine SDS Electrophoresis Buffer,10X
Tris/Borate Running Buffer	Alternative buffer system for discontinuous polyacrylamide gel electrophoresis	Tris - SDS - Boric Acid
Tris/Borate Sample Buffer	Alternative buffer system for discontinuous polyacrylamide gel electrophoresis	Tris - SDS - Bromophenol Blue - Sulfuric Acid - DTT (optional)
Tris/Borate Separating Gel Buffer	Alternative buffer system for discontinuous polyacrylamide gel electrophoresis	Tris - Sulfuric Acid
Tris/Borate Stacking Gel Buffer	Alternative buffer system for discontinuous polyacrylamide gel electrophoresis	Tris - Sulfuric Acid
Triton X-100 Extraction Buffer	Differential detergent lysis of cellular fractions	Triton X-100 - NaCl - PIPES pH 7.4 - MgCl₂ - PMSF - EDTA
Triton X-100 Lysis Buffer
Trypan Blue Stain	Assessment of membrane integrity for mammalian cells	Trypan Blue - PBS
Trypsin Solution	Digestion of tissue sections for antibody staining	Trypsin - CaCl2
TTBS	Blocking solution for Western blotting	Tris-Cl pH 7.5 - NaCl + TWEEN® 20
TTBS (ZFIN)	Blocking solution for Western blotting	Tris-Cl pH 7.5 - NaCl - TWEEN® 20
TTE, 10X		Tris-TAPS-EDTA, 10X
TWEEN 40/Deoxycholate Extraction Buffer	Differential detergent lysis of cellular fractions	TWEEN 40 - Sodium Deoxycholate - NaCl - PIPES pH 7.4 - MgCl₂ - PMSF - EDTA
Two-Dimensional Sample Buffer	Identification of novel protein kinase substrates	Urea - Thiourea - CHAPS - DTT
Tyrode's Solution		Tyrode's solution made from solid ingredients
Tyrode's Solution (from stock solutions)
UBE1 Reconstitution Buffer
Urea Loading Buffer, 2X	Electrophoresis of nucleic acids	Bromophenol Blue - Xylene Cyanol FF - Urea - EDTA
Urea Lysis Buffer	Two-dimensional gel electrophoresis	Urea - DTT - CHAPS - Carrier Ampholytes - Pefabloc
Urea/Glycerol Running Buffer	Alternative buffer system for discontinuous polyacrylamide gel electrophoresis	Tris - Glycine
Urea/Glycerol Sample Buffer	Alternative buffer system for discontinuous polyacrylamide gel electrophoresis	Tris - Urea - Bromophenol Blue - DTT (optional)
Urea/Glycerol Separating Gel Buffer	Alternative buffer system for discontinuous polyacrylamide gel electrophoresis	Tris - Glycerol
Urea/Glycerol Stacking Gel Buffer	Alternative buffer system for discontinuous polyacrylamide gel electrophoresis	Tris - Urea
Urethane Mixture	Preparation of mice for imaging	Urethane - NaCl
VALAP	Preparation of cells and tissues for fluorescence microscopy	Vaseline - Lanolin - Paraffin
Vitamin Stock Solution for Yeast X-gal Plates, 100X	Component of indicator media for lacZ^+/- S. cerevisiae	Thiamine - Biotin - Pyridoxine - Inositol - Pantothenic Acid
Vitamins Stock for Fission Yeast Media, 1000X	Component of synthetic medium for S. pombe	Nicotinic Acid - Inositol - Biotin - Pantothenic Acid
Wash Buffer	Immunoprecipitation	NaCl - EDTA - Triton X-100 - Tris-Cl pH 7.4
Wash Buffer	Tandem immunoaffinity purification using anti-FLAG and anti-HA antibodies	Tris-Cl pH 8.0 - KCl - MgCl₂ - Glycerol - TWEEN 20
Wash Buffer	Purification of intermediate filament proteins from bacterial inclusion bodies	Tris-Cl pH 8.0 - NaCl - EDTA - IGEPAL CA-630 - MgCl₂
Wash Buffer - Chromatin Immunoprecipitation	Chromatin immunoprecipitation	SDS - EDTA - BSA - Sodium Phosphate pH 7.2
Washing Buffer - Ribosome Display	Ribosome display	Tris-Acetate pH 7.5 - NaCl - Magnesium Acetate - TWEEN 20 - Heparin
Washing Solution - Phage Display	Phage display	Tris-Cl pH 7.5 - KCl - MgCl₂ - DTT - Glycerol - TWEEN® 20 . PMSF - Benzamidine - NaF - Na₃VO₄
X-gal plates for Lysed Yeast Cells on Filters	Media for assaying lacZ activity in S. cerevisiae	Agar - Na2HPO4 - NaH2PO4 - MgSO4
X-gal Reaction Buffer	LacZ staining in whole mount mouse embryos	Potassium Ferrocyanide - Potassium Ferricynide - MgCl₂ - IGEPAL CA-630 - Sodium Deoxycholate - PBS
X-gal Staining Solution	Immunohistochemistry of whole-mount mouse embryos	Sodium Phosphate pH 7.3 - Tris-Cl pH 7.3 - MgCl2 - Sodium Deoxycholate - IGEPAL CA-630 - Potassium Ferricyanide - Potassium Ferrocyanide - X-gal
X-gal Working Solution	LacZ staining of Drosophila	X-gal - FeNaP Buffer
Xpl Homogenization Buffer	Protein extraction from Arabidopsis	Sorbitol - HEPES pH 7.5 - EDTA - MgCl₂ - BSA - Sodium Ascorbate
Yeast Extract Medium (YE)	Rich medium for S. pombe	Yeast Extract - Glucose
Yeast Paste	Collection of Drosophila embryos	Dry Yeast - Water
Yeast X-gal Plates
Yeast X-gal Plates Solution I	Indicator medium for lacZ activity in S. cerevisiae	Phosphate Buffer Stock - Mineral Stock - Drop-Out Mix
Yeast X-gal Plates Solution II	Component of indicator media for lacZ+/- S. cerevisiae	Agar
YPAD (Slant Medium)	Preparation of slants for S. cerevisiae	Yeast Extract - Peptone - Glucose - Adenine - Agar
YPAD Broth	Rich medium for S. cerevisiae	Yeast Extract - Peptone - Glucose - Adenine Sulfate
YPD Agar	Rich medium for S. cerevisiae	Yeast Extract - Peptone - Glucose - Agar
YPD Broth	Rich medium for S. cerevisiae	Peptone - Yeast Extract - Glucose
YPDA	Rich medium for budding yeast	Yeast Extract - Dextrose - Peptone - Adenine
YPDG Medium
YPG	Solid, complex medium for S. cerevisiae, selection against petite mutants	Yeast Extract - Peptone - Glycerol - Agar
YRLB	Budding yeast	NaCl - Tris-Cl pH 7.5 - EDTA - SDS
Z Buffer	Two-hybrid systems	Sodium Phosphate - KCl - MgSO₄ - β-mercaptoethanol
α-Aminoadipate Plates	Solid medium for S. ceveisiae	YNB - Glucose - Lysine - Agar

Nectagen

Solutions

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