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Solutions
| Name | Use | Description |
|---|---|---|
| Sodium Glycine pH 8.8, 50 mM | ||
| Sodium Glycine pH 9.0, 50 mM | ||
| Sodium Glycine pH 9.2, 50 mM | ||
| Sodium Glycine pH 9.4, 50 mM | ||
| Sodium Glycine pH 9.6, 50 mM | ||
| Sodium Glycine pH 9.8, 50 mM | ||
| Sodium Oleate Solution, 1% (wt:vol) |
Additive for M. tuberculosis media. For oleic acid, 6% (vol:vol) equals 1% (wt:vol) |
|
| Sodium Phosphate pH 5.8, 100 mM | ||
| Sodium Phosphate pH 6.0, 1 M |
Sodium phosphate buffer |
NaH2PO4 - Na2HPO4 |
| Sodium Phosphate pH 6.0, 100 mM | ||
| Sodium Phosphate pH 6.1, 1 M |
NaH2PO4 - Na2HPO4 |
|
| Sodium Phosphate pH 6.2, 1 M |
Sodium phosphate buffer |
NaH2PO4 - Na2HPO4 |
| Sodium Phosphate pH 6.2, 100 mM | ||
| Sodium Phosphate pH 6.3, 1 M |
Sodium phosphate buffer |
NaH2PO4 - Na2HPO4 |
| Sodium Phosphate pH 6.4, 1 M |
Sodium phosphate buffer |
NaH2PO4 - Na2HPO4 |
| Sodium Phosphate pH 6.5, 1 M |
NaH2PO4 - Na2HPO4 |
|
| Sodium Phosphate pH 6.5, 100 mM | ||
| Sodium Phosphate pH 6.6, 1 M |
Sodium phosphate buffer |
NaH2PO4 - Na2HPO4 |
| Sodium Phosphate pH 6.6, 100 mM | ||
| Sodium Phosphate pH 6.7, 1 M |
Sodium phosphate buffer |
NaH2PO4 - Na2HPO4 |
| Sodium Phosphate pH 6.7, 100 mM | ||
| Sodium Phosphate pH 6.8, 1 M |
Sodium phosphate buffer |
NaH2PO4 - Na2HPO4 |
| Sodium Phosphate pH 6.8, 100 mM | ||
| Sodium Phosphate pH 6.9, 1 M |
Sodium phosphate buffer |
NaH2PO4 - Na2HPO4 |
| Sodium Phosphate pH 6.9, 100 mM | ||
| Sodium Phosphate pH 7.0, 1 M |
Sodium phosphate buffer |
NaH2PO4 - Na2HPO4 |
| Sodium Phosphate pH 7.0, 100 mM | ||
| Sodium Phosphate pH 7.1, 1 M |
NaH2PO4 - Na2HPO4 |
|
| Sodium Phosphate pH 7.1, 100 mM | ||
| Sodium Phosphate pH 7.2, 1 M |
Sodium phosphate buffer |
NaH2PO4 - Na2HPO4 |
| Sodium Phosphate pH 7.2, 100 mM | ||
| Sodium Phosphate pH 7.3, 100 mM | ||
| Sodium Phosphate pH 7.4, 100 mM | ||
| Sodium Phosphate pH 7.5, 100 mM | ||
| Sodium Phosphate pH 7.8, 100 mM | ||
| Sodium Phosphate pH 8.0, 100 mM | ||
| Solution D |
Isolation of total RNA from mammalian cells |
Guanidium Thiocyanate - Sodium Citrate - Sarkosyl - β-mercaptoethanol |
| Southern Blotting - Denaturing Solution |
Southern Blotting |
NaOH - NaCl |
| Southern Blotting - Equilibration Solution |
Southern blotting |
2X SSC |
| Southern Blotting - Neutralizing Solution |
Southern blotting |
NaCl - Tris-Cl pH 7.4 |
| Southern Blotting - Neutralizing Solution, High Salt |
Southern blotting |
NaCl - Tris-Cl pH 7.4 |
| Sperm Extender (ZFIN) | ||
| Sporulation Medium |
Sporulation of S. cerevesiae |
Potassium Acetate - Yeast Extract - Glucose - Agar |
| SSC, 20X |
Hybridization analysis of nucleic acids |
NaCl - Sodium Citrate pH 7.0 |
| SSCT (ZFIN) |
SST + TWEEN 20 0.1% (vol:vol) |
|
| SSPE, 20X |
Hybridization analysis of nucleic acids |
NaCl - NaH2PO4 - EDTA |
| SSTE |
Isolation of total RNA from plant tissue |
NaCl - SDS - Tris-Cl pH 8.0 - EDTA |
| Standard Buffer |
Imaging exocytosis |
NaCl - KCl - CaCl2 - MgCl2 - HEPES pH 7.2 |
| STE |
Tris-Cl pH 8.0 - EDTA - NaCl |
|
| STE, 10X |
NaCl - Tris-Cl pH 8.0 - EDTA |
|
| Sterile Cortex Buffer |
Specific examination of neuronal cells |
NaCl - KCl - Glucose - HEPES pH 7.4 - CaCl2 - MgSO4 |
| Sterile Glycerol Solution for Freezing Yeast Transformants |
Glycerol - MgSO4 - Tris-Cl pH 8.0 |
|
| STES Buffer |
Purification of high molecular weight DNA from yeast |
Tris-Cl pH 7.6 - NaCl - SDS - EDTA |
| STET |
NaCl - Tris-Cl pH 8.0 - EDTA - Triton X-100 |
|
| STM |
Saline Tris MgCl2 Buffer pH 8.0 |
|
| Stock Salts (ZFIN) | ||
| Stripping Buffer with SDS |
Removal of antibodies from Western blots before incubation with another antibody |
Tris-Cl pH 6.8 - SDS - β-mercaptoethanol |
| Stripping Mix A |
Peptide arrays |
Urea - SDS - PBS |
| Stripping Mix B |
Peptide arrays |
Acetic Acid - Ethanol |
| Subbing Solution |
Coating microscope slides and coverslips |
Gelatin - Chromium Potassium Sulfate |
| Subbing Solution |
Preparation of poly-lysine coated slides |
Gelatin - Chrome Alum (Chromium(III) Potassium Sulfate) - Sodium Azide - Polylysine - Avg. MW >300,000 |
| Sucrose-Proteinase K Cell Lysis Buffer |
Genomic DNA purification from mammalian cells |
Sucrose - SSC - EDTA - SDS - Proteinase K |
| Sulfuric Acid, 2 N | ||
| Super Broth (SB) |
Rich medium for E. coli |
Trypton - Yeast Extract - NaCl |
| Supplemented Buffer P |
Immobilized metal-ion affinity chromatography |
Sodium Phosphate pH 7.4 - NaCl - MgCl2 - Lysozyme - PMSF - Benzonase |
| Supplemented Minimal Medium (SMM) |
Synthetic media for S. cerevisiae |
SD - Supplements |
| Synthetic Dextrose |
Synthetic medium for budding yeast |
Dextrose - YNB - Ammonium Sulfate |
| Synthetic Dextrose Minimal Medium (SD) |
Solid synthetic media for S. cerevisiae |
YNB - Glucose - Agar |
| SYPRO® Orange Fluorescent Staining Solution |
Staining of proteins separated by SDS-PAGE |
SYPRO® Orange - Acetic Acid |
| SYPRO® Red Fluorescent Staining Solution |
Staining proteins separated by SDS PAGE |
SYPRO® Red - Acetic Acid |
| SYPRO™ Ruby Destain |
Destain of SYPRO Ruby-stained proteins transferred to nitrocellulose or PVDF membranes |
Tris-Cl pH 8.8 - Methanol |
| T4 DNA Polymerase Buffer, 10X |
Tris-Acetate pH 8.0 - Potassium Acetate - Magnesium Acetate - DTT - BSA |
|
| T4 Polynucleotide Kinase Buffer, 10X |
Phosphorylation of DNA iwth T4 polynucleotide kinase |
Tris-Cl pH 7.7 - MgCl2 - DTT - Spermidine HCl - EDTA |
| TAE, 50X |
DNA electrophoresis running buffer |
Tris - Acetate - EDTA |
| Tamoxifen, 60 mg/ml |
Induction of ER ligand binding domain - Cre recombinase fusion proteins in mice |
Tamoxifen - Ethanol - Peanut Oil |
| TB-Potassium Salts |
Component of Terrific Broth (TB), rich medium for E. coli |
K2HPO4 - KH2PO4 |
| TBE Electrophoresis Buffer, 5X |
DNA Electrophoresis |
Tris - Borate - EDTA |
| TBS |
Physiological buffer |
Tris-Cl pH 7.9 - NaCl |
| TBS (Ausubel) |
Physiological buffer |
NaCl - KCl - CaCl2 - Na2HPO4 - Tris Base - MgCl2 |
| TBS (Goldsmith and Berens) |
Tissue culture |
NaCl - Tris-Cl pH 7.4 |
| TBS (ZFIN) |
Tris-Cl pH 7.5 - NaCl |
|
| TBS + 1% BSA |
Blocking agent for Western blotting |
Tris-Cl pH 7.3 - NaCl - BSA |
| TBS + 1% BSA + 0.02% Sodium Azide |
Blocking agent for Western blotting |
Tris-Cl pH 7.3 - NaCl - BSA - Azide |
| TBS pH 7.5 |
Physiological buffer |
Tris-Cl pH 7.5 - NaCl |
| TBSB |
Preparation and immunolabeling of C. elegans |
TBS - BSA |
| TBST |
Blocking solution for Western blotting |
Tris-Cl pH 7.3 - NaCl - TWEEN® 20 |
| TE pH 8.0, 10X |
DNA storage |
Tris-Cl pH 8.0 - EDTA |
| TEA Solution |
Triethanolamine pH 11.5 - Triton X-100 - NaCl |
|
| TEM Fixative |
Transmission electron microscopy of Arabidopsis |
Glutaraldehyde - Paraformaldehyde - TWEEN® 20 |
| TEN |
Tris-Cl pH 8.0 - EDTA - NaCl |
|
| Terrific Broth (TB) | ||
| TES |
Selection of poly(A) RNA from total RNA by batch chromatography |
Tris-Cl pH 7.5 - EDTA - SDS |
| TES Buffer (ZFIN) | ||
| Testosterone Treatment Solution (ZFIN) | ||
| TEV Cleavage Buffer |
Tandem affinity chromatography |
Tris-Cl pH 8.0 - NaCl - IGEPAL CA-630 - EDTA - DTT |
| Thiosulfate Developing Solution |
Visualization of silver-stained proteins separated by SDS-PAGE |
Sodium Carbonate - Sodium Thiosulfate - Formaldehyde |
| Thiourea/Urea Lysis Buffer |
Two-dimensional gel electrophoresis |
Thiourea - Urea - CHAPS - DTT - Carrier Ampholytes - Pefabloc |
| Tissue Lysis Buffer |
Detergent lysis of animal tissues for immunoprecipitation |
KCl - Glycerol - Tris-Cl pH 8.0 - MgCl2 - TWEEN 20 |
| TM |
Tris MgCl2 Buffer pH 8.0 |
|
| TNE |
Purification of intermediate filament proteins from bacterial inclusion bodies |
Tris-Cl pH 8.0 - NaCl - EDTA |