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Sarkosyl Buffer |
Sarkosyl preparation of antigens from bacterial inclusion bodies
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Tris-Cl pH 7.4 - NaCl - ZnCl2 - β-mercaptoethanol - Sarkosyl
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SB Medium - Phage Display |
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Tryptone - Yeast Extract - MOPS
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Schonefeld Medium for Paramecia (ZFIN) |
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Scintillation Solution |
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SDS Buffer - Chemical Cross-linking |
Chemical chrosslinking with MBS
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Tris pH 6.8 - glycerol - β-mercaptoethanol - SDS - Coomassie Blue
|
|
SDS PAGE Resolving Gel, 10% Acrylamide |
Protein electrophoresis
|
Acrylamide (29:1) - Tris-Cl pH 8.8 - SDS - APS - TEMED
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|
SDS PAGE Resolving Gel, 12% Acrylamide |
Protein electrophoresis
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Acrylamide (29:1) - Tris-Cl pH 8.8 - SDS - APS - TEMED
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|
SDS PAGE Resolving Gel, 15% Acrylamide |
Protein electrophoresis
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Acrylamide (29:1) - Tris-Cl pH 8.8 - SDS - APS - TEMED
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|
SDS PAGE Resolving Gel, 5% Acrylamide |
Protein electrophoresis
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Acrylamide (29:1) - Tris-Cl pH 8.8 - SDS - APS - TEMED
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|
SDS PAGE Resolving Gel, 7.5% Acrylamide |
Protein electrophoresis
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Acrylamide (29:1) - Tris-Cl pH 8.8 - SDS - APS - TEMED
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|
SDS PAGE Stacking Gel |
Protein electrophoresis
|
Acrylamide (29:1) - Tris-Cl pH 6.8 - SDS - APS - TEMED
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|
SDS-Equilibration Buffer |
Identification of novel protein kinase substrates
|
Tris-Cl pH 8.8 - Urea - Glycerol - SDS - Bromophenol Blue - DTT
|
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SDS-PAGE Destaining Solution |
|
SDS-PAGE Destaining Solution
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SDS/Phosphate Running Buffer |
Alternative buffer system for discontinuous polyacrylamide gel electrophoresis
|
Sodium Phosphate pH 7.2 - SDS
|
|
SDS/Phosphate Sample Buffer |
Alternative buffer system for discontinuous polyacrylamide gel electrophoresis
|
Sodium Phosphate pH 7.2 - SDS - Glycerol - Bromophenol Blue - DTT (optional)
|
|
SDS/Phosphate Separating Gel Buffer |
Alternative buffer system for discontinuous polyacrylamide gel electrophoresis
|
Sodium Phosphate pH 7.2 - SDS
|
|
Second-strand Buffer, 10X |
Isolation and amplification of RNA from single cells and small amounts of tissue
|
Tris-Cl pH 7.4 - KCl - MgCl2 - Ammonium Sufate
|
|
Semi-Dry Transfer Buffer |
Semi-dry transfer of proteins to membranes for Western blottting
|
Tris Base - Glycine - SDS - Methanol
|
|
Semi-Dry Transfer Buffer, 25X without Methanol |
Semi-dry transfer of proteins to membranes for Western blottting
|
Tris Base - Glycine - SDS
|
|
Shoot-Inducing Medium |
Genetic manipulation of Arabidopsis
|
Gamborg's B5 Salts - Glucose - MES pH 5.7 - Agar - 2-iP - IAA - Kanamycin - Carbenicillin
|
|
Silver Staining - Developer Solution |
Visualization of silver-stained proteins in SDS-PAGE gels
|
Sodium Carbonate - Formaldehyde
|
|
Silver Staining - Fixation Solution |
Fixation of proteins in SDS-PAGE gels for silver staining
|
Ethanol - Acetic Acid
|
|
Silver Staining - Quenching Solution |
Quenching development of silver-staining of proteins in SDS-PAGE gels
|
Acetic Acid
|
|
Silver Staining - Silver Solution |
Silver staining of proteins in SDS-PAGE gels
|
AgNO3
|
|
Slice Solution |
Ballistic delivery of dyes to neurons
|
NaCl - KCl - NaH2PO4 - MgCl2 - CaCl2 - Glucose - NaH2CO3
|
|
SM |
Storage and dilution of bacteriophage λ stocks
|
NaCl - MgSO4 - Tris-Cl pH 7.5 - Gelatin
|
|
SNET |
Purification of high molecular weight DNA by spooling
|
Tris-Cl pH 8.0 - EDTA - NaCl - SDS
|
|
Soaking Solution - For Charged Membranes |
Hybridization analysis of nucleic acids
|
NaCl - NaOH
|
|
Soaking Solution - For Uncharged Membranes |
Hybridization analysis of nucleic acids
|
NaCl - NaOH
|
|
SOB Medium |
Rich medium for E. coli
|
Tryptone - Yeast Extract - NaCl - KCl - MgCl2
|
|
SOC Medium |
|
SOC Medium
|
|
Sodium Acetate pH 3.6, 100 mM |
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Sodium Acetate pH 3.8, 100 mM |
|
|
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Sodium Acetate pH 4.0, 100 mM |
|
|
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Sodium Acetate pH 4.0, 2 M |
RNA isolation from cultured cells or tissues
|
Sodium Acetate - Acetic Acid (2 M with respect to sodium ions)
|
|
Sodium Acetate pH 4.2, 100 mM |
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Sodium Acetate pH 4.4, 100 mM |
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Sodium Acetate pH 4.6, 100 mM |
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Sodium Acetate pH 4.8, 100 mM |
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Sodium Acetate pH 5.0, 100 mM |
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Sodium Acetate pH 5.2, 100 mM |
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Sodium Acetate pH 5.4, 100 mM |
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Sodium Acetate pH 5.6, 100 mM |
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|
|
Sodium Barbital Chloride pH 6.8, 50 mM |
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Sodium Barbital Chloride pH 7.0, 50 mM |
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Sodium Barbital Chloride pH 7.2, 50 mM |
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Sodium Barbital Chloride pH 7.4, 50 mM |
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Sodium Barbital Chloride pH 7.6, 50 mM |
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|
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Sodium Barbital Chloride pH 7.8, 50 mM |
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Sodium Barbital Chloride pH 8.0, 50 mM |
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Sodium Barbital Chloride pH 8.2, 50 mM |
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Sodium Barbital Chloride pH 8.4, 50 mM |
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Sodium Barbital Chloride pH 8.6, 50 mM |
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|
|
Sodium Barbital Chloride pH 8.8, 50 mM |
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Sodium Barbital Chloride pH 9.0, 50 mM |
|
|
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Sodium Barbital Chloride pH 9.2, 50 mM |
|
|
|
Sodium Borohydride, 0.5 mg/ml in PBS |
Fixation of attached cells
|
Sodium Borohydride - PBS
|
|
Sodium Cacodylate pH 5.0, 50 mM |
|
|
|
Sodium Cacodylate pH 5.2, 50 mM |
|
|
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Sodium Cacodylate pH 5.4, 50 mM |
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|
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Sodium Cacodylate pH 5.6, 50 mM |
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|
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Sodium Cacodylate pH 5.8, 50 mM |
|
|
|
Sodium Cacodylate pH 6.0, 50 mM |
|
|
|
Sodium Cacodylate pH 6.2, 50 mM |
|
|
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Sodium Cacodylate pH 6.4, 50 mM |
|
|
|
Sodium Cacodylate pH 6.6, 50 mM |
|
|
|
Sodium Cacodylate pH 6.8, 50 mM |
|
|
|
Sodium Cacodylate pH 7.0, 50 mM |
|
|
|
Sodium Cacodylate pH 7.0, 50 mM |
|
|
|
Sodium Cacodylate pH 7.2, 50 mM |
|
|
|
Sodium Cacodylate pH 7.4, 50 mM |
|
|
|
Sodium Carbonate-Bicarbonate pH 10.1, 100 mM |
|
|
|
Sodium Carbonate-Bicarbonate pH 10.3, 100 mM |
|
|
|
Sodium Carbonate-Bicarbonate pH 10.5, 100 mM |
|
|
|
Sodium Carbonate-Bicarbonate pH 10.8, 100 mM |
|
|
|
Sodium Carbonate-Bicarbonate pH 9.2, 100 mM |
|
|
|
Sodium Carbonate-Bicarbonate pH 9.4, 100 mM |
|
|
|
Sodium Carbonate-Bicarbonate pH 9.5, 100 mM |
|
|
|
Sodium Carbonate-Bicarbonate pH 9.8, 100 mM |
|
|
|
Sodium Carbonate-Bicarbonate pH 9.9, 100 mM |
|
|
|
Sodium Citrate pH 3.0, 50 mM |
|
|
|
Sodium Citrate pH 3.2, 50 mM |
|
|
|
Sodium Citrate pH 3.4, 50 mM |
|
|
|
Sodium Citrate pH 3.6, 50 mM |
|
|
|
Sodium Citrate pH 3.8, 50 mM |
|
|
|
Sodium Citrate pH 4.0, 50 mM |
|
|
|
Sodium Citrate pH 4.2, 50 mM |
|
|
|
Sodium Citrate pH 4.4, 50 mM |
|
|
|
Sodium Citrate pH 4.6, 50 mM |
|
|
|
Sodium Citrate pH 4.8, 50 mM |
|
|
|
Sodium Citrate pH 5.0, 50 mM |
|
|
|
Sodium Citrate pH 5.2, 50 mM |
|
|
|
Sodium Citrate pH 5.4, 50 mM |
|
|
|
Sodium Citrate pH 5.6, 50 mM |
|
|
|
Sodium Citrate pH 5.8, 50 mM |
|
|
|
Sodium Citrate pH 6.0, 50 mM |
|
|
|
Sodium Citrate pH 6.2, 50 mM |
|
|
|
Sodium Glycine pH 10.4, 50 mM |
|
|
|
Sodium Glycine pH 10.6, 50 mM |
|
|
|
Sodium Glycine pH 8.6, 50 mM |
|
|
