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Solutions
| Name | Use | Description |
|---|---|---|
| Phosphate-Buffer Stock Solution for Yeast X-gal Plates, 10X |
Component of indicator media for lacZ+/- S. cerevisiae |
KH2PO4 - Ammonium Sulfate - KOH |
| Phosphate-Citrate Buffer pH 2.6 | ||
| Phosphate-Citrate Buffer pH 2.8 | ||
| Phosphate-Citrate Buffer pH 3.0 | ||
| Phosphate-Citrate Buffer pH 3.2 | ||
| Phosphate-Citrate Buffer pH 3.4 | ||
| Phosphate-Citrate Buffer pH 3.6 | ||
| Phosphate-Citrate Buffer pH 3.8 | ||
| Phosphate-Citrate Buffer pH 4.0 | ||
| Phosphate-Citrate Buffer pH 4.2 | ||
| Phosphate-Citrate Buffer pH 4.4 | ||
| Phosphate-Citrate Buffer pH 4.6 | ||
| Phosphate-Citrate Buffer pH 4.8 | ||
| Phosphate-Citrate Buffer pH 5.0 | ||
| Phosphate-Citrate Buffer pH 5.2 | ||
| Phosphate-Citrate Buffer pH 5.4 | ||
| Phosphate-Citrate Buffer pH 5.6 | ||
| Phosphate-Citrate Buffer pH 5.8 | ||
| Phosphate-Citrate Buffer pH 6.0 | ||
| Phosphate-Citrate Buffer pH 6.2 | ||
| Phosphate-Citrate Buffer pH 6.4 | ||
| Phosphate-Citrate Buffer pH 6.6 | ||
| Phosphate-Citrate Buffer pH 6.8 | ||
| Phosphate-Citrate Buffer pH 7.0 | ||
| Pipette Solution |
Ballistic delivery of dyes to neurons |
Potassium Gluconate - KCl - MgCl2 - HEPES - EGTA - CaCl2 - ATP - GTP - Glucose |
| PK Buffer, 2X |
Use and labeling of GST fusions |
Potassium Phosphate pH 7.15 - MgCl2 - NaF - DTT |
| PK Buffer, 2X |
RNAi in Drosophila |
Tris-Cl pH 7.5 - EDTA - NaCl - SDS |
| PNK Buffer, 10X |
Buffer for phosphorylation of DNA with polynucleotide kinase |
Tris-Cl pH 7.6 - MgCl2 - DTT |
| Ponceau S Stain, 10X |
Reversible staining of proteins immobilized on membranes |
Ponceau S - TCA - Sulfosalicylic Acid |
| Ponceau Solution |
Reversible staining of proteins transferred to membranes |
Ponceau S - Acetic Acid |
| Potassium Acetate Solution |
Isolation of total DNA from blood |
Potassium Acetate - Acetic Acid |
| Potassium Phosphate pH 5.8, 1 M | ||
| Potassium Phosphate pH 5.8, 100 mM |
Buffer solution |
K2HPO4 - KH2PO4 |
| Potassium Phosphate pH 6.0, 1 M | ||
| Potassium Phosphate pH 6.0, 100 mM |
Buffer solution |
K2HPO4 - KH2PO4 |
| Potassium Phosphate pH 6.2, 1 M | ||
| Potassium Phosphate pH 6.2, 100 mM |
Buffer solution |
K2HPO4 - KH2PO4 |
| Potassium Phosphate pH 6.4, 1 M | ||
| Potassium Phosphate pH 6.4, 100 mM |
Buffer solution |
K2HPO4 - KH2PO4 |
| Potassium Phosphate pH 6.6, 1 M | ||
| Potassium Phosphate pH 6.6, 100 mM |
Buffer solution |
K2HPO4 - KH2PO4 |
| Potassium Phosphate pH 6.8, 1 M | ||
| Potassium Phosphate pH 6.8, 100 mM |
Buffer solution |
K2HPO4 - KH2PO4 |
| Potassium Phosphate pH 7.0, 1 M | ||
| Potassium Phosphate pH 7.0, 100 mM |
Buffer solution |
K2HPO4 - KH2PO4 |
| Potassium Phosphate pH 7.2, 1 M | ||
| Potassium Phosphate pH 7.2, 100 mM |
Buffer solution |
K2HPO4 - KH2PO4 |
| Potassium Phosphate pH 7.4, 1 M | ||
| Potassium Phosphate pH 7.4, 100 mM |
Buffer solution |
K2HPO4 - KH2PO4 |
| Potassium Phosphate pH 7.6, 1 M | ||
| Potassium Phosphate pH 7.6, 100 mM |
Buffer solution |
K2HPO4 - KH2PO4 |
| Potassium Phosphate pH 7.8, 1 M | ||
| Potassium Phosphate pH 7.8, 100 mM |
Buffer solution |
K2HPO4 - KH2PO4 |
| Potassium Phosphate pH 8.0, 1 M | ||
| Potassium Phosphate pH 8.0, 100 mM |
Buffer solution |
K2HPO4 - KH2PO4 |
| Powdered Supplement Mixture for SC Medium |
Component of synthetic media for S. cerevesiae |
Adenine - Ala - Arg - Asn - Asp - Cys - Gln - Glu - Gly - His - Inositol - Ile - Leu - Lys - Met - PABA - Phe - Pro - Ser - Thr - Trp - Tyr - Ura - Val |
| Prehybridization Mix |
Southern blotting |
Formamide - Dextran Sulfate - NaCl - Tris-Cl pH 7.5 - EDTA - Torula RNA - Denhardt's |
| Prehybridization Solution |
Hybridization analysis of nucleic acids |
Sodium Phosphate pH 7.2 - SDS - EDTA |
| Prehybridization Solution |
Southern blotting |
SSC - Denhardt's - SDS - Denatured Salmon Sperm DNA |
| Presporulation Medium |
Sporulation of S. cerevesiae |
Yeast Extract - Peptone - Glucose - Agar |
| Protease Inhibitor Mix for Glycosylation Experiments, 100X |
Protein preparation |
Leupeptin - Antipain - Benzamidine - Aprotinin - Chymostatin - Pepstatin |
| Protease Inhibitor Mix, 100X |
Protein preparation |
Benzamidine - Bacitracin - Aprotinin - Pepstatin - Leupeptin |
| Protein Elution Buffer |
X-ray crystallography |
HEPES pH 7.5 - NaCl - DTT - PMSF |
| Protein Lysate Buffer |
X-ray crystallography |
HEPES pH 7.5 - NaCl - DTT - PMSF |
| Protein Transfer Buffer |
Western blotting |
Tris - Glycine - SDS - Methanol |
| Proteinase Digestion Buffer |
In situ peptide mapping |
Tris-Cl pH 6.8 - SDS - Glycerol - Bromophenol Blue |
| Proteinase Digestion Buffer |
RNA purification from mammalian cells |
Tris-Cl pH 8.0 - EDTA - NaCl - SDS |
| Proteinase K Digest Buffer |
Dot and slot blotting of nucleic acids |
Tris-Cl pH 8.0 - EDTA |
| Proteinase K Stock Solution (CSHL) | ||
| Proteinase K Stop Solution, 10 X |
Dot and slot blotting for nucleic acids |
Glycine - PBS |
| Proteinase Solution |
In situ peptide mapping |
Tris-Cl pH 6.8 - SDS - Gycerol - Bromophenol Blue - Proteinase |
| Quenching Solution |
FRET analysis |
Tris-Cl pH 8.0 - NaCl |
| Quick DNA Prep Extraction Buffer |
Purification of high molecular weight DNA from plant cells |
Tris-Cl pH 7.5 - NaCl - EDTA - SDS |
| Rapid Coomassie Blue Staining Solution |
Staining of proteins separated by SDS-PAGE |
Acetic Acid - Coomassie Brilliant Blue G-250 |
| RBC Lysis Buffer |
Isolation of total DNA from red blood cells |
Tris-Cl pH 7.6 |
| Rearing Solution |
Rearing Xenopus |
NaCl - KCl - CaNO3 - MgSO4 - HEPES pH 7.4 |
| Refolding Buffer - IMAC |
Immobilized metal-ion affinity chromatography |
Imidazole - NaCl - βME - Tris |
| Renilla Luciferase Assay Buffer |
Protein fragment complementation assays |
KCl - NaCl - HEPES pH 7.4 - DTT - MgCl2 - EDTA - IGEPAL CA-630 |
| Rescue Buffer |
Two-hybrid systems |
Tris-Cl pH 7.5 - EDTA - β-mercaptoethanol |
| Resuspension Buffer 1 |
Isolation and amplification of probes for yeast DNA microarrays |
Tris-Cl pH 8.0 - EDTA |
| Resuspension Buffer 2 |
Isolation and amplification of probes for yeast DNA microarrays |
Tris-Cl pH 8.0 - EDTA |
| Reswelling Buffer |
Two-dimensional gel electrophoresis |
Urea - DTT - CHAP -Pharmalyte |
| Ringer's Solution - Calcium Free (ZFIN) | ||
| Ringer's Solution - High Calcium (ZFIN) | ||
| Ringer's Solution - Normal (ZFIN) | ||
| RIPA Buffer |
Chromatin immunprecipitation |
HEPES-KOH pH 7.6 - LiCl - EDTA - IGEPAL CA-630 - Sodium Deoxycholate |
| RIPA Buffer |
Radioimmunoprecipitation assay buffer |
|
| RIPA Buffer - Chromatin Immunoprecipitation |
Chromatin immunoprecipitation |
Tris-Cl pH 8.0 - EDTA - EGTA - Triton X-100 - Sodium Deoxycholate - SDS - NaCl - PMSF |
| RNA Amplification Buffer, 10X |
Isolation and amplification of RNA from single cells and small amounts of tissue |
Tris-Cl pH 7.5 - MgCl2 - NaCl - Spermidine |
| RNA Extraction Buffer - RNase Inhibitor |
RNA purification from mammalian cells |
NaCl - MgCl2 - Tris-Cl pH 8.6 - IGEPAL CA-630 - DTT - RNase Inhibitor |
| RNA Extraction Buffer - Vanacyl-Ribonucleoside Complexes |
RNA purification from mammalian cells |
NaCl - MgCl2 - Tris-Cl pH 8.6 - IGEPAL CA-630 - DTT - Vanadyl-Ribonucleoside Complexes |
| RNA Gel-loading Buffer |
Nuclease S1 RNA protection |
Formamide - Bromophenol Blue - Xylene Cyanol FF - EDTA - SDS |
| RNA Loading Buffer, 10X |
Loading buffer for RNA electrophoresis |
Gliycerol - EDTA - Bromophenol Blue - yelen Cyanol FF |
| RNA Precipitation Solution |
Isolation of total RNA from plant tissue |
Sodium Citrate - NaCl |
| RNA Resuspension Solution |
RNA isolation and RT-PCR |
Tris-Cl pH 7.9 - NaCl - MgCl2 |
| RNase Buffer |
RNA digestion with RNase |
Tris-Cl pH 8.0 - NaCl - DTT |
| S30 Buffer, 100X | ||
| Saline/EDTA Buffer Plus Glucose |
Isolation of germ cells and tissue culture |
EDTA - NaCl - KCl - Na2HPO4 - KH2PO4 - Phenol Red - Glucose
|
| SAM, 30 mM |
Methylation |
S-(5′-Adenosyl)-L-methionine |
| Sample Buffer |
Two-dimensional isoelectric focusing/SDS-polyacrylamide gel electrophoresis |
Urea - IGEPAL CA-630 - β-mercaptoethanol |