Solutions

Name	Use	Description
MB Medium	Synthetic medium for S. pombe	KH2PO4 - MgSO4 - NaCl - CaCl2 - Ammonium Sulfate - Glucose - Potassium Acetate - Minerals Stock - Vitamin Stock
MBP Binding Buffer	Preparation of MBP-fusion proteins from bacteria	Tris-Cl pH 7.4 - NaCl - EDTA
MBP Elution Buffer	Preparation of MBP-fusion proteins from bacteria	Tris-Cl pH 7.4 - NaCl - EDTA - Maltose
MBP Lysis Buffer	Preparation of MBP-fusion proteins from bacteria	Tris-Cl pH 7.4 - NaCl - EDTA - cOmplete Protease Inhibitor Cocktail, EDTA-free
MBS pH 6.5		MOPS-buffered saline pH 6.5
MBS pH 6.5 - CaCl2		MOPS pH 6.5 - NaCl - CaCl2
MBS Salts, 10X	Component of Modified Barth's Saline for Xenopus	NaCl - KCl - MgSO4 - HEPES pH 7.8 - NaHCO3
MBS-T pH 7.4		MOPS buffered Saline with 0.05% Tween 20 pH 7.4
MCB pH 7.2		Maleimide Coupling Buffer
MCB-Cys pH 7.2		Maleimide Conjugation Buffer with 10 ug/ml L-Cys
MCB-TCEP pH 7.2		Maleimide Coupling Buffer with 1 mM TCEP
MDM2 Buffer	NMR imaging	MES-d13 - KCl - DTT-d10 - NaN₃
MEM/PVP Medium	RNAi in mouse oocytes and early embryos	Bicarbonate-free MEM - Sodium Pyruvate - Gentamicin - PVP - HEPES pH 7.2
MEMFA	Culture medium for Xenopus oocytes	MOPS pH 7.4 - EGTA - MgSO4 - Formaldehyde
MEMFA	Imaging fixed tissue	Formaldehyde - MOPS pH 7.6 - EDTA - MgSO4
Methanol/Acetic Acid Destaining Solution	Destaining of Coomassie-stained proteins separated by SDS-PAGE	Methanol - Acetic Acid
Methylene Blue Solution	Staining agarose gels	Methylene Blue - Sodium Acetate
Mineral Stock Solution for Yeast X-gal Plates, 1000X	Component of indicator media for lacZ+/- S. cerevisiae	FeCl3 - MgSO4
Minerals Stock for Fission Yeast Media, 10,000X	Component of minimal synthetic medium fo S. pombe	H₃BO₃ - MnSO₄ - ZnSO₄ - FeCl₃ - MoO₄ - KI - CuSO₄ - Citric Acid
Minimal Sporulation Medium	Sporulation of S. cerevesiae	Potassium Acetate - Agar
Modified Barth's Saline (MBS)	Modified Barth's Saline for Xenopus	CaCl2 - NaCl - KCl - MgSO4 - HEPES pH 7.8 - NaHCO3
Modified Frog Ringer's (MR)	Ringer's solution for Xenopus	NaCl - KCl - CaCl₂ - MgCl₂ - HEPES pH 7.6
MOPS Buffer, 10X	Hybridization analysis of nucleic acids	MOPS pH 7.0 - Sodium Acetate - EDTA
MOPS Running Buffer, 10X	Agarose gel electrophoresis of RNA	MOPS pH 7.0 - Sodium Acetate - EDTA
Mounting Medium	Mounting samples for microscopy	Glycerol - Boric Acid pH 9.0 - N-propylgallate
Mouse ACSF	Artificial Cerebrospinal Fluid. Imaging actin from slices of transgenic mouse brain	NaCl - KCl - HEPES pH 7.4 - CaCl₂ - MgCl₂ - Glucose
Mowiol Mounting Solution		Glycerol - Mowiol® 4-88 - H2O - Tris-Cl pH 8.5
MS-222 Anesthetic Solution	Anesthetizing Xenopus	MS-222 - Rearing Solution
MTLn3 Growth Medium	Xenogfraft culture	MEM α - Nucleosides - FBS - Pen/Strep
MTSB, 2X	Visualization of microtubules	PIPES pH 6.8 - EGTA - PEG 6000 - Azide - MgSO4
Murray's Clearing Medium		Benzyl Benzoate/Benzyl Alcohol, BB/BA
MWB		Maleimide Wash Buffer
n-Dodecyl-β-D-Maltoside - Cholesterol Hemisuccinate Tris
n-Propyl Gallate Antifade Medium	Preparation of cells and tissues for fluorescence microscopy	n-propyl gallate - Glycerol
Na-TES	Selection of poly(A) RNA from total RNA by batch chromatography	Tris-Cl pH 7.5 - EDTA - SDS - NaCl
NaCl/Ethanol Solution	Purification of high molecular weight DNA by spooling	NaCl - Ethanol
NBT-BCIP Reaction Buffer	Detection of alkaline phosphatase	NBT - BCIP
NBT/BCIP Reaction Buffer	Alkaline phosphatase staining	NBT - BCIP
NETN	Coimmunoprecipitation	NaCl - EDTA - Tris-Cl pH 8.0 - IGEPAL CA-630
Neutralization/Binding Solution	Purification of plasmid DNA with silica membrane spin columns	Guanidine HCl - Potassium Acetate pH 4.2
NGM	RNAi in C. elegans. Worm culture medium.	NaCl - Agar - Peptone - Cholesterol - CaCl₂ - MgSO₄ - Potassium Phosphate pH 6.0
Nick Translation Buffer, 10X		Tris-Cl pH 7.5 - MgSO₄ - BSA - DTT
Nitrocellulose Stripping Buffer	Southern blotting	SSC - EDTA
Non-Denaturing IMAC Buffer	Renaturation and elution of native His-tagged proteins bound to Ni-NTA columns	Sodium Phosphate pH 8.0 - NaCl
Non-Denaturing IMAC Buffer - 250 mM Imidazole	Renaturation and elution of native His-tagged proteins bound to Ni-NTA columns	Sodium Phosphate pH 8.0 - NaCl - Imidazole
Normal Amphibian Medium (NAM)
Normal Frog Ringer's	Imaging synaptic vesicles	NaCl - KCl - CaCl2 - HEPES pH 7.2
NRB1		Nectagen Resuspension Buffer 1
NRB2		Nectagen Resuspension Buffer 2
NTM	In situ hybridization of intact mouse embryos with RNA probes	NaCl - Tris-Cl pH 9.5 - MgCl₂
NTMT	In situ hybridization of intact mouse embryos with RNA probes	NaCl - Tris-Cl pH 9.5 - MgCl₂ - TWEEN® 20
Nuclear Lysis Buffer	Chromatin immunoprecipitation	Tris-Cl pH 8.0 - EDTA - SDS - Protease Inhibitors
Nuclease S1 Buffer, 10X	Isolation and amplification of RNA from single cells and small amounts of tissue	NaCl - Sodium Acetate pH 4.5 - ZnSO4
Nuclease S1 Digestion Buffer	Nuclease S1 RNA protection	NaCl - Sodium Acetate pH 4.5 - ZnSO4
Nuclease S1 Stop Mixture	Nuclease S1 RNA protection	Ammonium Acetate - EDTA - Carrier RNA
Nylon Membrane Stripping Buffer I	Southern blotting	Tris-Cl pH 8.0 - EDTA - Denhardt's
Nylon Membrane Stripping Buffer II	Southern blotting	SSPE - Formamide
NZCYM Medium	Rich media for E. coli	N-Z Amine - NaCl - Casamino Acids - Yeast Extract - MgSO₄
NZM Medium	Rich media for E. coli	N-Z Amine - NaCl - MgSO4
NZYM Medium	Rich media for E. coli	N-Z Amine - NaCl - Yeast Extract - MgSO₄
OCR-2	Culture medium for Xenopus oocytes	NaCl - KCl - CaCl₂ - MgCl₂ - Na₂HPO₄ - HEPES - NaOH
One-Phor-All Buffer Plus, 10X	Preparation of nucleic acid for array hybridization analysis	Tris-Acetate pH 7.5 - Magnesium Acetate - Potassium Acetate
OPI Supplement, 100X	Supplement to support the growth of hybridoma and meyeloma cells at low densities	Oxaloacetic Acid - Sodium Pyruvate - Insulin
Paraformaldehyde Fixative - 8% Stock	Fixation of cells before imaging	Paraformaldehyde - PBS
Paraformaldehyde Fixative Solution	FRET analysis	PBS - Paraformaldehyde
Paraformaldehyde, 4% (wt:vol) in PBS	Preparation of frozen tissue sections	Paraformaldehyde - PBS
Paramecia Medium Stock Solution I (ZFIN)
Paramecia Medium Stock Solution II (ZFIN)
Paramecia Storage Medium - Long Term (ZFIN)
Paraphenylenediamine Mounting Medium	Preparation of cells and tissues for fluorescence microscopy	Glycerol - PBS, 0.1 X - p-phenylenediamine - Sodium Carbonate - Sodium Bicarbonate
PB	Labeling in vitro with phosphorylated precursors
PBF Percoll	Protein extraction from Arabidopsis	PEG 4000 - BSA - Ficoll - Percoll
PBS (Ausubel)		NaCl - KCl - Na₂HPO₄ - KH₂PO₄
PBS (Dulbecco and Vogt)		NaCl - KCl - Na₂HPO₄ - KH₂PO₄ - CaCl₂ - MgCl₂
PBS (Galfred and Milstein)	PBS for hybridoma culture	NaCl - KCl - Na₂HPO₄ - KH₂PO₄
PBS (Kuchler)	PBS with higher pH modified for trypsination	NaCl - KCl - Na2HPO4 - KH2PO4 - Glucose
PBS + 5% Milk		Phosphate buffered saline with 5% non-fat dry milk
PBS RJS		Phosphate buffered saline, pH 7.4
PBS without Potassium pH 7.2		NaCl - Sodium Phosphate pH 7.2
PBS, 10X		NaCl - KCl - Sodium Phosphate pH 7.4
PBSMT	Immunohistochemistry of whole-mount mouse embryos	PBS - Milk - Triton X-100
PBST		PBS - TWEEN 20
PBST + 5% Milk	Blocking agent	PBS - TWEEN 20 - Milk
PBST RJS	Blocking agent	PBS - TWEEN 20
PBT	Immunohistochemistry of whole-mount mouse embryos	PBS - BSA - Triton X-100
PCR Buffer	Genotyping ES cells	Ammonium Sulfate - Tris-Cl pH 8.8 - MgCl₂ - β-mercaptoethanol - EDTA
PCR Extraction Buffer (ZFIN)
PCR Lysis Solution A	Purification of high molecular weight DNA by spooling	Tris-Cl ph 8.8 - Ammonium Sulfate - β-mercaptoethanol - MgCl₂ - EDTA - SDS - Proteinase K
PCR Lysis Solution B	Purification of high molecular weight DNA by spooling	TrisCl pH 8.3 - KCl - MgCl2 - IGEPAL CA-630 - TWEEN 20 - Proteinase K
PCR Reaction Buffer with MgCl2, 10X	DNA amplification by PCR	Tris-Cl pH 8.3 - KCl - Triton X-100 - MgCl2
PCR Reaction Buffer without MgCl2, 10X	DNA amplification by PCR	Tris-Cl pH 8.3 - KCl - Triton X-100
PEB		Polyol Elution Buffer
PEG-NaCl
PEM	S. pombe	PIPES pH 6.9 - EGTA - MgSO₄
Pembal	S. pombe	PIPES pH 6.9 - EGTA - MgSO4 - BSA - Azide - Lysine
Permeabilizing FACS Buffer	Intracellular staining by flow cytometry	PBS - FBS - Sodium Azide - Saponin
PHEM Buffer pH 6.9	Microscopy	PIPES pH 6.9 - HEPES - EGTA - MgCl2
PHEM Buffer pH 6.9, 2 X	Microscopy	PIPES pH 6.9 - HEPES - EGTA - MgCl2
PHEM pH 5.9, 4X		PIPES - HEPES - EDTA - Magnesium Buffer pH 5.9
Phenol:Chloroform	Extraction of protein from nucleic acid preparations	Phenol:Chloroform

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