Solutions

Name	Use	Description
Hybridization Buffer	Nuclease S1 RNA protection	PIPES pH 6.4 - EDTA - NaCl
Hybridization Buffer	Nuclease S1 RNA protection	PIPES pH 6.8 - EDTA - NaCl - Formamide
Hybridization Buffer	RNAse protection	PIPES pH 6.4 - EDTA - NaCl - Formamide
Hybridization Buffer (Aqueous)	Southern blotting	Sodium Phosphate pH 6.8 - NaCl - MgCl2 - Denhardt's
Hybridization Buffer (Chromatin Immunoprecipitation)	Chromatin immunoprecipitation	SDS - EDTA - BSA - Sodium Phosphate pH 7.2
Hybridization Buffer - 20% Formamide	Southern blotting, low stringency	Formamide - SSC - SDS - EDTA - Denhardt's
Hybridization Buffer - 25% Formamide	Southern blotting, medium stringency	Formamide - SSC - SDS - EDTA - Denhardt's
Hybridization Buffer - 37.5% Formamide	Southern blotting, high stringency	Formamide - SSC - SDS - EDTA - Denhardt's
Hybridization Buffer - 50% Formamide	Southern blotting, very high stringency	Formamide - SSC - SDS - EDTA - Denhardt's
Hybridization Solution	Southern blotting	SSC - Dextran Sulfate - SDS - Denatured Salmon Sperm DNA
Hydroxylamine Buffer	Antibody labeling with maleimido dye	Hydroxylamine - EDTA - PBS
Hypotonic Buffer	RNAi in Drosophila	MgCl₂ - Potassium Acetate
Hypotonic Lysis Buffer	Identification of novel protein kinase substrates	HEPES - EGTA - MgCl₂
Hypotonic Lysis Buffer	Selection of polysomal RNA	KCl - MgCl₂ - Tris-Cl pH 7.4 Cycloheximide
IEF Buffer	Coimmunoprecipitation of FLAG-epitope -tagged proteins	Urea - DTT - Pharmalyte - Triton X-100
IGEPAL CA-630 Lysis	Tissue/Cell Lysis	NaCl - IGEPAL CA-630 - Tris-Cl pH 8.0
IGEPAL Lysis Buffer
IM, 10 X	Labeling in vitro with phosphorylated precursors
Imidazole Chloride pH 6.2, 50 mM
Imidazole Chloride pH 6.4, 50 mM
Imidazole Chloride pH 6.6, 50 mM
Imidazole Chloride pH 6.8, 50 mM
Imidazole Chloride pH 7.0, 50 mM
Imidazole Chloride pH 7.2, 50 mM
Imidazole Chloride pH 7.4, 50 mM
Imidazole Chloride pH 7.6, 50 mM
Imidazole Chloride pH 7.8, 50 mM
Immobilization Buffer	Electron microscopy	PBS - TWEEN® 20 - TCEP
In Situ Hybridization Staining Buffer (ZFIN)
Inclusion Body Lysis Buffer	Sarkosyl preparation of antigens from bacterial inclusion bodies	PBS - EDTA - DTT - cOmplete Protease Inhibitor Cocktail, EDTA-free
Inclusion Body Wash Buffer	Immobilized metal-ion affinity chromatography	Urea - NaCl - Triton X-100 - Tris-Cl pH 8.0
Infiltration Medium		For Agrobacterium
IPG DryStrip Rehydration Buffer (Urea + Thiourea)	Two-dimensional gel electrophoresis	Urea - Thiourea - DTT - CHAPS - Pharmalyte
IPG DryStrip Rehydration Buffer (Urea)	Two-dimensional gel electrophoresis	Urea - DTT - CHAPS - Pharmalyte
IPP150 Buffer	Tandem affinity chromatography	Tris-Cl pH 8.0 - NaCl - IGEPAL CA-630
IPP150 Calmodulin Elution Buffer	Tandem affinity chromgatography	Tris-Cl pH 8.0 - NaCl - IGEPAL CA-630 - Magnesium Acetate - Imidazole - EGTA - β-mercaptoethanol
IPP150 Calmodulin-Binding Buffer	Tandem affinity chromgatography	Tris-Cl pH 8.0 - NaCl - IGEPAL CA-630 - Magnesium Acetate - Imidazole - CaCl2 - β-mercaptoethanol
Isopropanol Fixing Solution	Fixation of proteins separated by SDS-PAGE	Isopropanol - Acetic Acid
Ketamine-Xylazine-Acepromazine Cocktail	Anesthetization of mice	Ketamine - Xylazine - Acepromazine
KFI, 10X	Isolation and amplification of RNA from single cells and small amounts of tissue	Tris-Cl pH 7.5 - MgCl2 - NaCl - DTT
Killing Buffer		For killing bacteria before RNA harvest
Kinase Buffer, 10X	Identification of novel protein kinase substrates	HEPES pH 7.4 - Magnesium Acetate - DTT
Klenow Buffer, 10X	Reaction buffer for DNA pol I Klenow fragment	Tris-Cl pH 7.6 - MgCl₂
KLH Buffer	Cross-linking peptides to KLH with maleimide	Sodium Phosphate pH 7.2 - NaCl - EDTA - Sucrose
Krebs-Henseleit Buffer Modified	Basal salt mixture for tissue culture	Glucose - MgSO₄ - KH₂PO₄ - KCl - NaCl
KSOM Culture Medium	In vitro culturing of mouse embryonic stem cells	NaCl - KCl - KH₂PO₄ - MgSO₄ - Glucose - Penicillin G - Streptomycin - Sodium Lactate - NaHCO₃ - Phenol Red - Sodium Pyruvate - CaCl₂ - EDTA - L-Glutamine - BSA
KSOM Medium	RNAi in mouse oocytes and early embryos	NaCl - KCl - KH2PO4 - MgSO4 - CaCl2 - NaHCO3 - Sodium Lactate - Sodium Pyruvate - L-glutamine - EDTA - BSA - Gentamicin - Glucose
L-Cysteine pH 8.5, 50 mM	Blocking of sulfhydryl reactive groups after conjugation	L-Cysteine HCl
Label Buffer - Electron Microscopy	Electron microscopy	PBS - BSA
Labeling Buffer, 10X - Phage Display	Phage display	Hepes pH 7.0 - MgCl₂ - MnCl₂ - DTT - BSA
Laemmli Sample Buffer, 1X	SDS polyacrylamide gel electrophoresis of proteins	SDS - Glycerol - Tris-Cl pH 6.8 - Bromophenol Blue
Laemmli Sample Buffer, 2X
Laemmli Sample Buffer, 5X
Lambda Diluent	Dilution of bacteriophage λ	Tris-Cl pH 7.5 - MgSO₄
Lambda Dilution Buffer	Diluting bacteriophage λ	Tris-Cl pH 7.5 - NaCl - MgCl₂ - Gelatin
Lauryl Maltose Neopentyl Glycol - Cholesterol Hemisuccinate Tris
LB	Rich liquid medium for E. coli	Tryptone - Yeast Extract - NaCl. Luria-Bertani Media
LiCl Buffer - Chromatin Immunoprecipitation	Chromatin immunoprecipitation	LiCl - IGEPAL CA-630 - Sodium Deoxycholate - EDTA - Tris-Cl pH 8.0
Ligase Buffer - Chromatin Immunoprecipitation	Chromatin immunoprecipitation	MgCl2 - DTT - ATP Tris-Cl pH 7.6
Ligation Buffer + ATP, 2X	Ligation of DNA	Tris-Cl pH 7.6 - MgCl2 - DTT - BSA - ATP
Ligation Buffer, 10X	DNA ligation	Tris-Cl pH 7.6 - MgCl₂ - DTT - BSA
Low Calcium Buffer	Imaging exocytosis	NaCl - KCl - CaCl₂ - MgCl₂ - HEPES- EGTA pH 7.2
Low Salt Buffer (HSB)	Recovery of unincorporated dyes by HPLC	Triethylammonium Acetate pH 8.0 - Acetonitrile
Low Stringency Hybridization Buffer	Northern blotting	NaH₂PO₄ - NaH₂PO₄ - EDTA - SDS
Lowry Reagent A	Component of Lowry protein concentration assay	Copper-Tartrate-Carbonate - SDS - NaOH
Lowry Reagent B	Component of Lowry protein concentration assay	Folin-Ciocalteu phenol
Luminol Visualization Solution	Visualization of immunoblots with HRP-labeled antibodies	Luminol - p-Iodophenol - Tris-Cl pH 7.5 - H2O2
Lysate Buffer	Purification of high molecular weight DNA by spooling	Tris-Cl pH 8.8 - Ammonium Sulfate - BSA
Lysis Buffer	Electron microscopy	Tris-Cl pH 7.5 - NaCl - IGEPAL CA-630 - PMSF - cOmplete™ Protease Inhibitor Cocktail - Na₃VO₄ - NaF
Lysis Buffer	Isolation and amplification of probes for yeast DNA microarrays	Sorbitol - Tris-Cl pH 8.0 - EDTA
Lysis Buffer	Coimmunoprecipitation of FLAG-epitope -tagged proteins	Triton X-100 - Glycerol - NaCl - Tris-Cl pH 7.5 - EDTA - Protease Inhibitors
Lysis Buffer	Preparation of Drosophila embryo extracts	DTT - HEPES-KOH pH 7.4 - Magnesium Acetate - Pefabloc - Potassium Acetate
Lysis Buffer	RNA isolation and RT-PCR	Guanidine Thiocyanate - Tris -Cl pH 7.4 - β-mercaptoethanol
Lysis Buffer	Protein fragment complementation assays	Tris-Cl pH 7.6 - NaCl - SDS - EDTA - PMSF
Lysis Buffer 1	Chromatin immunprecipitation	HEPES-KOH pH 7.5 - NaCl - EDTA - Glycerol - IGEPAL CA-630 - Triton X-100
Lysis Buffer 1 - Yeast	Lysis of yeast cells for protein extraction	Tris-Cl pH 7.5 - KCL - EDTA - IGEPAL CA-630 - Glycerol
Lysis Buffer 1 Containing Protease Inhibitors - Yeast	Lysis of yeast cells for protein extraction	Tris-Cl pH 7.5 - KCL - EDTA - IGEPAL CA-630 - Glycerol - DTT - PMSF - Leupeptin - Chymostatin - Pepstatin - Aprotinin
Lysis Buffer 2	Chromatin immunoprecipitation	Tris-Cl pH 8.0 - NaCl - EDTA - EGTA
Lysis Buffer 2 - Yeast	Detergent-free lysis of yeast cells for protein extracts	Tris-Cl pH 7.5 - NaCl - EDTA
Lysis Buffer 3	Chromatin immunprecipitation	Tris-Cl pH 8.0 - NaCl - EDTA - EGTA - Sodium Deoxycholate - Sarkosyl
Lysis Buffer for a 24-well Plate	Purification of high molecular weight DNA from mouse	Tris-Cl pH 8.5 - EDTA - SDS - NaCl - Proteinase K
Lysis Buffer for a 96-well Plate	Purification of high molecular weight DNA from mouse	Tris-Cl pH 7.5 - EDTA - NaCl - Sarkosyl - Proteinase K
Lysis Buffer for GST Pulldown	Use and labeling of GST fusions	Tris-Cl pH 8.0 - NaCl - EDTA - IGEPAL CA-630 - Protease Inhibitors
Lysis Solution - Yeast	Two-hybrid systems	Zymolase - Rescue Buffer (Tris-Cl pH 7.5 - EDTA - β-mercaptoethanol)
Lysozyme Solution	Isolation of total RNA from E. coli	Tris - EDTA - Lysozyme
M16 Culture Medium	In vitro culturing of mouse embryonic stem cells	NaCl - KCl - CaCl₂ - KH₂PO₄ - MgSO₄ - NaHCO₃ - Sodium Lactate - Sodium Pyruvate - Glucose - BSA - Penicillin G - Streptomycin Sulfate - Phenol Red
M16 Culture Medium - Calcium Free	In vitro culturing of mouse embryonic stem cells	NaCl - KCl - KH₂PO₄ - MgSO₄ - NaHCO₃ - Sodium Lactate - Sodium Pyruvate - Glucose - BSA - Penicillin G - Streptomycin Sulfate - Phenol Red
M2 Culture Medium	In vitro culturing of mouse embryonic stem cells	NaCl - KCl - CaCl₂ -KH₂PO₄ - MgSO₄ - NaHCO₃ - HEPES - Sodium Lactate - Sodium Pyruvate - Glucose - BSA - Penicillin G - Streptomycin Sulfate - Phenol Red
M2 Culture Medium - Calcium Free	In vitro culturing of mouse embryonic stem cells	NaCl - KCl - KH2PO4 - MgSO4 - NaHCO3 - HEPES - Sodium Lactate - Sodium Pyruvate - Glucose - BSA - Penicillin G - Streptomycin Sulfate - Phenol Red
M2 Lysis Buffer	Identification of novel protein kinase substrates	Tris-Cl pH 7.4 - NaCl - Glycerol - Triton X-100 - EDTA - EGTA - NaF - β-Glycerophosphate - Tetrasodium Pyrophosphate - Na₃VO₄ - Aprotinin - Leupeptin - PMSF
M9 Buffer	Preparation and immunolabeling of C. elegans	KH2PO4 - Na2HPO4 - NaCl - NH4Cl
M9 Media	Minimal medium for E. coli	Na₂HPO₄ - KH₂PO4 - NH₄Cl - NaCl
M9 Medium (for Imaging)	LIve imaging of late-stage embryos/larvae from mouse, chick, Xenopus and C. elegans	Potassium Phosphate Monobasic - Sodium Phosphaste Dibasic - NaCl - MgSO₄
M9 Salts + CaCl2, 5X	Minimal medium for E. coli	Na₂HPO₄ - KH₂PO4 - NH₄Cl - NaCl - CaCl₂
M9 Salts, 5X		M9 minimal salts, 5X concentrated stock
M9, 10X	RNAi in C. elegans	KH₂PO₄ - K₂HPO₄ - NaCl - MgSO₄
MACS Buffer for Cell Separation	Separation of mammalian cells with magnetic beads	PBS - BSA - EDTA
MAL Indicator Medium	Solid indicator medium for maltose^+/-S. cerevisiae	Yeast Extract - Peptone - Maltose - Bromocresol Purple - Agar
Maleic Acid Buffer	Dot and slot blotting of nucleic acids	Maleic Acid - NaCl
Malt Extract Agar (ME)	Rich media for S. pombe	Malt Extract - Agar

Nectagen

Solutions

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