Solutions

Name	Use	Description
Elution Buffer	Chromatin immunoprecipitation	Tris-Cl pH 8.0 - EDTA - SDS
Elution Buffer	Selection of poly(A) RNA from total RNA by oligo dT	Tris-Cl pH 7.6 - EDTA - SDS
Elution Buffer - IMAC	Immobilized metal-ion affinity chromatography	Sodium Phosphate pH 7.4 - NaCl - High Imididazole
Embryo LCMR	Dissection of Xenopus embryos	NaCl - KCl - CaCl2 - MgCl2 - HEPES pH 7.6
Embryo Wash	Collection of Drosophila embryos	Triton X-100 - NaCl
Enzyme Diluent	Dilution of enzymes	Tris-Cl pH 7.5 - BSA - 2-ME
EPON (ZFIN)
Equilibration Buffer - 2D Gel Electrophoresis	Two-dimensional gel electrophoresis	Urea - Glycerol - SDS - Tris-Cl pH 8.8
Ethidium Bromide Assay Solution	DNA/RNA concentration assay	Ethidium Bromide - Tris-Cl pH 7.4 - NaCl - EDTA
Explant LCMR	Dissection of Xenopus explants	NaCl - KCl - CaCl2 - MgCl2 - HEPES pH 7.6
Extraction Buffer	Imaging intermediate filament proteins in living cells	Urea - Tris-Cl Ph 7.4 - EGTA - β-ME - PMSF
Extraction Buffer (Plant RNA)	Isolation of total RNA from plant tissue	CTAB - PVP K 30 - Tris-Cl pH 8.0 - EDTA - NaCl - Spermidine - β-mercaptoethanol
Extraction Buffer for E. coli Lysates	Two-dimensional gel electrophoresis	Urea - CHAPS - Tris Base - MgCl₂
Extraction Buffer for Eukaryotic Lysates	Two-dimensional gel electrophoresis	Urea - CHAPS - IPG Buffer - DTT
Extraction Solution for Rat Liver Protein Extract	Two-dimensional gel electrophoresis	Urea - Thiourea - ASB-14 - Pharmalyte
FACS Buffer	Intracellular staining by flow cytometry	PBS - FBS - Sodium Azide
Fe/NaP Buffer for X-gal Staining	LacZ staining of Drosophila	Sodium Phosphate pH 7.2 - Potassium Ferrocyanide - Potassium Ferricyanide - NaCl - MgCl2
First-strand cDNA Buffer	DNA microarray	Tris-Cl pH 8.3 - KCl - MgCl2
Fish Water (ZFIN)
Fix Buffer (ZFIN), 2X
Fixative for Frozen Sections	Immunohistochemistry of whole-mount mouse embryos	Paraformaldehyde - PIPES - MgCl₂ - EGTA
Formaldehyde Fixing Solution	Fixation of proteins separated by SDS-PAGE	Methanol - Formaldehyde
Formaldehyde Gel Running Buffer, 5X
Formaldehyde Gel-Loading Buffer	Hybridization analysis of nucleic acids	Glycerol - EDTA - Bromophenol Blue - Xylene Cyanol FF
Formaldehyde Solution (11%)	Chromatin immunoprecipitation	HEPES-KOH pH 7.5 - NaCl - EDTA - EGTA - Formaldehyde
Formamide Denaturation Buffer	Purification of high molecular weight DNA	Tris-Cl pH 8.0 - NaCl - Formamide
Fragmentation Buffer	Labeling mRNA with biotin nucleotides	Tris-Acetate pH 8.1 - Magnesium Acetate - Potassium Acetate
GAL Indicator Medium	<p>Indicator medium for galactose <sup>+/- </sup><em>S. cerevisiae</em></p>	Yeast Extract - Peptone - Agar - Bromothymol Blue
Gel Elution Buffer	Nuclease S1 RNA protection	Ammonium Acetate - EDTA - SDS
Gel Filtration Buffer	X-ray crystallography	HEPES pH 7.5 - NaCl - DTT
Gelatin Solution	FRET analysis	PBS - Gelatin
Gelvatol	Polyvinyl alcohol - Glycerol - PBS - Sodium Azide
Germination Medium	Genetic manipulation of Arabidopsis	MS Salts - Sucrose - MES pH 5.7 - Agar - Gamborg's Vitamins
GHCL Buffer (ZFIN)		Guanidine HCl Buffer pH 7.0
Ginsburg Fish Ringer's (ZFIN)
GIT Buffer (ZFIN)		Guanidine Isothiocyanate Buffer pH 7.5
Glutaraldehyde, 1% (wt:vol) in PBS	Fixation of attached cells	Glutaraldehyde - PBS
Glycerol Antifade Mounting Medium	Preparation of cells and tissues for fluorescence microscopy	Propyl Gallate - DABCO - p-Phenyenediamine - Glycerol
Glycerol Tolerant Gel Buffer, 20X
Glycine Chloride pH 2.0, 0.1 M
Glycine Chloride pH 2.2, 50 mM
Glycine Chloride pH 2.4, 50 mM
Glycine Chloride pH 2.6, 50 mM
Glycine Chloride pH 2.8, 50 mM
Glycine Chloride pH 3.0, 50 mM
Glycine Chloride pH 3.2, 50 mM
Glycine Chloride pH 3.4, 50 mM
Glycine Chloride pH 3.6, 50 mM
Glyoxal/DMSO Gel - Agarose, 1% (wt:vol) + 1X Sodium Phosphate Running Buffer	Separation of RNA in glyoxal/DMSO-containing gels	Agarose - Sodium Phosphate Running Buffer - Soium Iodoacetate
Glyoxal/DMSO Loading Buffer	RNA electrophoresis with glyoxal/DMSO-containing gels	Sodium Phosphate pH 7.0 - Glycerol - Bromophenol Blue - Xylene Cyanol FF
Gradient Buffer	Selection of polysomal RNA	KCl - MgCl2 - Tris-Cl pH 7.4
Grape Juice Agar Plate	Collection of Drosophila embryos	Agar - Sucrose - Grape Juice - Methyl-4-hydroxybenzoate
Grinding Buffer	Nuclear extraction from Arabidopsis	Glycerol - Sucrose - Tris-Cl pH 8.0 - PMSF
GST Elution Buffer	Preparation of GST-fusion proteins from bacteria	Tris-Cl pH 8.0 - Reduced Glutathione - EDTA
GST Lysis Buffer	Preparation of GST-fusion proteins from bacteria	PBS - EDTA - DTT - cOmplete EDTA-free Protease Inhibitors
Guanidine Chloride Buffer (for Preparation of RNA)	Sodium Citrate pH 7.0 - Guanidine Chloride - DTT - SDS - DEPC	RNA preparation from mammalian cells
Guanidine HCl Homogenization Buffer I	RNA purification from mammalian cells	Guanidine HCl - Sodium Acetate - DTT - Sodium Lauryl Sarcosinate
Guanidine HCl Homogenization Buffer II	RNA purification from mammalian cells	Guanidine HCl - Sodium Acetate - DTT - EDTA
Guanidinium Thiocyanate Homogenization Buffer	RNA purification from mammalian cells	Guanidium Thiocyanate - Tris-Cl pH 7.5 - β-mercaptoethanol
Guanidium Thiocyanate Hoogenization Buffer	Isolation of total RNA by ultracentrifugation	Guanidium Thiocyanate - Tris-Cl pH 7.5 - β-mercaptoethanol
Guianidine-Chloride Stripping Buffer	Removal of antibodies from Western blots before incubation with another antibody	GnHCl - TWEEN 20 - β-mercaptoethanol
GUS Staining Solution	Histochemistry of GUS fusion	Sodium Phosphate pH 7.0 - EDTA - Triton X-100 - Potassium Ferrocyanide - Potassium Ferricyanide - X-Gluc
GYT Medium		GYT Medium (Tung and Chow 1995)
Hank's Balanced Salt Solution		HBSS made from solid ingredients
Hank's Balanced Salt Solution (from stock solutions)		HBSS made from stock solutions
HBS	Cytoskeleton visualization	NaCl - Sodium Phosphate Dibasic - KCl - CaCl₂ - HEPES pH 7.5
HBS (Ausubel)	Transfection	NaCl - KCl - Glucose Na₂HPO₄ - HEPES
HBS (Dittmar)		NaCl - HEPES pH 7.4
HBS (Liu and Blumenthal)	Liposome preparation	NaCl - KCl - HEPES pH 7.3
HDM2 Buffer 1	NMR imaging	Potassium Phosphate - EDTA - DTT
HDM2 Buffer 2	NMR imaging	HEPES pH 7.0 - NaCl - DTT
HEMGN Buffer	Hydrophobic interaction chromatography	HEPES - EDTA - MgCl2 - Glycerol - IGEPAL CA-630 - DTT
Heparin IV Infusion	Catheterization of mice	Heparin - NaCl
HEPES Protein Extraction Buffer	Protein extraction (Solution A)	HEPES pH 7.4 - CaCl2 - PMSF - Protease Inhibitors
HEPES-buffered Tyrode's Solution	Cytoskeleton visualization	NaCl - KCl - HEPES pH 7.4 - CaCl₂ - MgCl₂- Glucose
High MW DNA Purification - Dialysis Buffer 1	Purification of high molecular weight DNA	Tris-Cl pH 8.0 - NaCl - EDTA
High MW DNA Purification - Dialysis Buffer 2	Purification of high molecular weight DNA	Tris-Cl pH 8.0 - NaCl - EDTA
High Salt Buffer (HSB)	Recovery of unincorporated dyes by HPLC	Triethylammonium Acetate pH 8.0 - Acetonitrile
High-salt Modified Barth's Saline (High-salt MBS)	High Salt Modified Barth's Saline for Xenopus	CaCl₂ - NaCl - KCl - MgSO₄ - HEPES pH 7.8 - NaHCO₃
His-Fusion Binding Buffer	Preparation of His-fusion proteins from bacteria	Sodium Phosphate pH 7.4 - NaCl - Imidazole
His-Fusion Elution Buffer	Preparation of His-fusion proteins from bacteria	Sodium Phosphate pH 7.4 - NaCl - Imidazole
His-Fusion Lysis Buffer	Preparation of His-fusion proteins from bacteria	Sodium Phosphate pH 7.4 - NaCl - Imidazole - cOmplete Protease Inhibitor Cocktail, EDTA-free
Hoechst 33258 Assay Solution	DNA concentration assay	Hoechst 3328 - Tris-Cl pH 7.4 - NaCl - EDTA
Homemade Chemiluminescent Substrate Solution for HRP: Solution A	Chemiluminescent detection of horseradish peroxidase	Tris-Cl pH 7.6 - Sodium Luminol
Homemade Chemiluminescent Substrate Solution for HRP: Solution B	Chemiluminescent detection of horseradish peroxidase	p-Coumaric Acid - DMSO
Homogenization Buffer	Purification of intermediate filament proteins from bacterial inclusion bodies	Tris-Cl pH 8.0 - Sucrose - EDTA
Homogenization Buffer	Imaging intermediate filament proteins in living cells	Tris-Cl pH 7.4 - MgCl₂ - β-mercaptoethanol - PMSF
Homogenization Buffer (DNA)	Genomic DNA purification from mammalian cells	Tris-Cl pH 7.5 - NaCl - EDTA - Spermine - Spermidine - Sucrose
Homogenization Buffer (RNA) with Proteinase K	RNA purification from mammalian cells	Tris-Cl pH 7.5 - NaCl - EDTA - SDS - Proteinase K
Homogenization Buffer - Arabidopsis	Protein extraction from Arabidopsis	Tris-Acetate pH 7.9 - Potassium Acetate - EDTA - DTT - Glycerol - Protease Inhibitors
Homogenization Buffer A	Protein extraction from mammalian cells	Tris-Cl pH 7.5 - EDTA - NaCl - DTT
Homogenization Buffer B (Glycerol)	Protein extraction from mammalian cells	Tris-Cl pH 7.5 - Glycerol - Magnesium Acetate - EDTA -DTT - PMSF
Homogenization Buffer B (Sucrose)	Protein extraction from mammalian cells	Tris-Cl pH 7.5 - Sucrose - Magnesium Acetate - EDTA -DTT - PMSF
Homogenization Buffer I (No Proteinase K)	RNA purification from mammalian cells	Tris-Cl pH 9.0 - EDTA - SDS
Howard Ringer's Solution	Preparation of chick embryos	NaCl - CaCl₂ - KCl
HPLC Separation Buffer A	Mass spectrometry	Acetonitrile - Formic Acid
HPLC Separation Buffer B	Mass spectrometry	Acetonitrile - Formic Acid
HPLC Separation Buffer C	Mass spectrometry	Acetonitrile - Formic Acid - Ammonium Acetate
*HYB (ZFIN)**
HYB+ (ZFIN)

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Solutions

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