Solutions

Name	Use	Description
Buffer A (FPLC Mono Q)	Purification of proteins and inhibitors from cell culture	Tris-Cl pH 7.2 - DTT
Buffer B (FPLC Mono Q)	Purification of proteins and inhibitors from cell culture	Tris-Cl pH 7.2 - NaCl - DTT
Buffered Saline, 10X
Callus-Inducing Medium	Genetic manipulation of Arabidopsis	Gamborg's B5 Salts - Glucose - MES pH 5.7 - Agar - 2,4-D, Kinetin
CAPS Transfer Buffer	Western blotting	CAPS
Carbonate Developing Solution	Visualization of silver-stained proteins separated by SDS-PAGE	Formaldehyde - Sodium Carbonate
Carbonate-Bicarbonate Buffer	Preparation of Paraphenylenediamine Mounting Medium	Sodium Carbonate - Sodium Bicarbonate
Carnoy's Fixative	Sample fixation	Ethanol - Chloroform - Acetic Acid
Cartilage Staining Solution (ZFIN)
Cell Lysis Buffer	Isolation of total DNA from blood	Tris-Cl pH 8.0 - EDTA - SDS
Cell Lysis Buffer	Purification of high molecular weight DNA by spooling	Ttris-Cl pH 7.5 - NaCl - EDTA - Sarkosyl - Proteinase K
Cell Lysis Solution	Purification of high molecular weight DNA by spooling	Guanidium HCl - Sodium Acetate
Cell Resuspension Buffer	Genomic DNA purification	Tris-Cl pH 7.4 - NaCl - MgCl₂
Chick ACSF
Cholesterol Hemisuccinate Tris - CHAPS
CIP Dephosphorylation Buffer, 10X	Dephosphoryation of DNA with calf intestinal phosphatase	ZnCl₂ - MgCl₂ - Tris-Cl pH 8.3
Citrate Binding Buffer	Preparation of mFc- and hFc-rusion proteins from mammalian cells	Sodium Citrate - NaCl - EDTA
Citrate Elution Buffer	Preparation of mFc- and hFc-rusion proteins from mammalian cells	Sodium Citrate - NaCl - EDTA
Citrate-Buffered Saline	Peptide arrays	Sodium Citrate pH 7.0 - NaCl - KCl
Cleavage Buffer - Hydroxylamine Fragmentation	In situ peptide mapping by hydroxylamine fragmentation	Hydroxylamine HCl - Guanidine HCl - Potassium Carbonate pH 9.0
CMFM (ZFIN)
Color Developing Solution	Peptide arrays	Citrate Buffered Saline - MTT - BCIP - MgCl₂
Column Buffer	Imaging intermediate filament proteins in living cells	Urea - Sodium Phosphate pH 7.4 - NaCl - DTT - PMSF
Column Buffer	Buffer exchange of nucleic acid solutions with gel filtration	Tris-Cl pH 8.0 - EDTA - SDS
Column Buffer	Purification of intermediate filament proteins from bacterial inclusion bodies	Tris-Cl pH 8.0 - Urea - EDTA - DTT
Column Regeneration Buffer	Hydrophobic interaction chromatography	Tris - EDTA - NaCl - IGEPAL CA-630 pH 7.6
Column Storage Buffer	Hydrophobic interaction chromgatography	Tris - EDTA - NaCl - Azide pH 7.6
Column-Loading Buffer, 2X	Selection of poly(A) RNA from total RNA by oligo dT	Tris-Cl pH 7.6 - NaCl - EDTA - Sarkosyl
Compromise Restriction Buffer (B-ME), 10X	Restriction digestion of DNA	Tris-Cl pH 8.0 - MgCl₂ - NaCl - β-mercaptoethanol
Compromise Restriction Buffer (DTT), 10X	Restriction digestion of DNA	Tris-Cl pH 8.0 - MgCl₂ - NaCl - DTT
Coomassie Brilliant Blue Destain	Destaining Coomassie-stained protein in SDS PAGE gels	Methanol - Acetic Acid
Coomassie Brilliant Blue Destain - High Methanol	Destaining Coomassie-stained protein in SDS PAGE gels	Methanol - Acetic Acid
Coomassie Brilliant Blue Destain - Low Methanol	Destaining Coomassie-stained protein in SDS PAGE gels	Methanol - Acetic Acid
Coomassie Brilliant Blue Staining Solution	Protein staining in SDS PAGE gels	Coomassie Brilliant Blue R250 - Methanol - Acetic Acid
Copper Tartrate Carbonate (CTC)	Lowry protein concentration assay	Copper Sulfate - Potassium Tartrate - Sodium Carbonate
Cortland's Salt Solution (ZFIN)
Cross-Linking Buffer A	Cross-linking antibodies to beads with imidoesters	Tris-Cl pH 8.0 - NaCl - EDTA - IGEPAL CA-630
Cryoprotective Agent	Cryopreservation of mouse sperm	Raffinose - Skim milk
Crystal Harvest Buffer	X-ray crystallography	mPEG 5000 - Bis-Tris Methane pH 6.5
CTAB DNA Extraction Buffer	Purification of high molecular weight DNA from plant cells	Tris-Cl pH 8.0 - EDTA - NaCl - CTAB - PVP 40000
Cytoskeleton Solubilization Buffer (Non-Reducing)
Cytoskeleton Solubilization Buffer (Reducing)
CZB Medium	RNAi in mouse oocytes and early embryos	NaCl - KCl - KH₂PO₄ - MgSO₄ - CaCl₂ - NaHCO₃ - Sodium Lactate - Sodium Pyruvate - L-glutamine - EDTA - BSA - Gentamicin - Phenol Red
DAB Heavy Metal Stain (ZFIN)
DAB Solution (ZFIN)
DAB Substrate Solution for HRP	Chromogenic detection of horseradish peroxidase	Tris-Cl pH 7.6 - DAB Tetrahydrochloride - NiCl2
Danieau's Solution, 30X
DB		Denaturing Buffer, pH 8.5
Decalcifying Solution	Decalcification of tissues for paraffin embedding	Formic Acid - HCl
Denaturing Buffer Containing Guanidine Hydrochloride	Size exclusion chromatography	Urea - DTT - NaCl - Tris pH 7.5
Denaturing Buffer Containing Urea	Size exclusion chromatography	Urea - DTT - NaCl - Tris pH 7.5
Denaturing IMAC Buffer	Purification of denatured 6-His tagged proteins	NaH₂PO₄ - Tris-Cl - Guanidine HCl
Denaturing IMAC Buffer - 250 mM Imidazole	Purification of denatured 6-His tagged proteins	NaH₂PO₄ - Tris-Cl - Guanidine HCl - Imidazole
Denaturing IMAC Buffer - EDTA	Purification of denatured 6-His tagged proteins	NaH₂PO₄ - Tris-Cl - Guanidine HCl - EDTA
Denaturing IMAC Buffer - TCEP	Purification of denatured 6-His tagged proteins	NaH₂PO₄ - Tris-Cl - Guanidine HCl - TCEP
Denaturing Lysis Buffer	Denaturling lysis of cells before immunoprecipitation	Tris-Cl pH 7.5 - SDS
Denhardt's Reagent, 100X	Blocking reagent for nucleic acid hybridization	Ficoll - PVP - BSA
Denhardt's Reagent, 50X	Blocking agent for Southern blotting	Ficoll - PVP-40 - BSA
Dent's Fix	Imaging fixed tissue	Methanol - DMSO
Depolarizing Buffer	Imaging exocytosis	NaCl - KCl - CaCl₂ - MgCl₂ - HEPES pH 7.2
Deprotection Mix	Peptide arrays	TFA - TIBS - H₂O - DCM
Detergent Buffer	Purification of intermediate filament proteins from bacterial inclusion bodies	Tris-Cl Ph 8.0 - NaCl - EDTA - IGEPAL CA-630 - Deoxycholate
Detergent Rinse	Immunohistochemistry of whole-mount mouse embryos	Sodium Phosphate pH 7.3 - MgCl₂ - Sodium Deoxycholate - IGEPAL CA-630
Developing Solution	Mass spectrometry	Na₂CO₃ - Formaldehyde - Na₂S₂O₃
Developing Solution (for AP)	ELISA with alkaline phosphatase conjugate	DEA Substrate Buffer - pNPP
Dialysis Buffer	Purification of intermediate filament proteins from bacterial inclusion bodies	Sodium Phosphate pH 7.4 - β-mercaptoethanol - PMSF
Dialysis Buffer	Purification of proteins and inhibitors from cell culture	Tris-Cl pH 7.2 - DTT - Glycerol
Diaminobenzidine Solution	Staining of hydrogen peroxide conjugates	DAB - H₂O₂ - Tris-Cl pH 7.6
Dietrich's Fixative (ZFIN)
Digestion Buffer	Isolation of mouse genomic DNA from mouse tails	NaCl - Tris-Cl pH 8.0 - EDTA - SDS
Digestion Buffer - Mass Sectrometry	Mass spectrometry	NH₄HCO₃ - CaCl₂
Digitonin Extraction Buffer	Differential detergent lysis of cellular fractions	Digitonin - NaCl - PIPES pH 6.8 - MgCl₂ - EDTA - PMSF
Dilution Buffer - Chromatin Immunoprecipitation	Chromatin immunoprecipitation	Tris pH 8.0 - EGTA - Triton X-100 - NaCl - Protease Inhibitors
Dimethyl Pimelimidate Solution	Hydrophobic interaction chromatography	DMP - Triethanolamine
Disruption Buffer	Purification of proteins and inhibitors from cell culture	HEPES pH 7.2 - EDTA - DTT - Pepstatin - Leupeptin - Chymostatin
Dissasembly Buffer	Purification of intermediate filament proteins from bacterial inclusion bodies	Urea - Sodium Phosphate pH 7.2 - β-mercaptoethanol - PMSF
DNA Dilution Buffer	DNA hybridization analysis	SSC - Salmon Sperm DNA
DNA Extraction Buffer	Isolation of total RNA from fixed tissues	Tris-Cl pH 7.6 - EDTA - TWEEN 20 - Proteinase K
DNA Extraction Buffer (ZFIN)
DNA Gel-Loading Buffer Type I, 6X	Loading dye for electrophoresis	Bromophenol Blue - Xylene Cyanol FF - Sucrose
DNA Gel-Loading Buffer Type II, 6X	Loading dye for electrophoresis	Bromophenol Blue - Xylene Cyanol FF - Ficoll
DNA Gel-Loading Buffer Type III, 6X	Loading dye for electrophoresis	Bromophenol Blue - Xylene Cyanol FF - Glycerol
DNA Gel-Loading Buffer Type IV, 6X	Loading dye for electrophoresis	Bromophenol Blue - Sucrose
DNase I Buffer, 5X	Isolation of total RNA from E. coli	MgCl₂ - Tris-Cl pH 7.5 - EDTA - DTT
DNase RQ1 Buffer, 10X
DNase Storage Buffer
Dounce Homogenization - Buffer A	Cell lysis by Dounce homogenization	HEPES-KOH pH 7.9 - KCl - MgCl2 - DTT - PMSF
Dounce Homogenization - Buffer B	Cell lysis by Dounce homogenization	HEPES-KOH pH 7.9 - KCl - MgCl2
Dounce Homogenization - Buffer C	Cell lysis by Dounce homogenization	HEPES-KOH pH 7.9 - NaCl - Glycerol - MgCl2 - EDTA - DTT - PMSF
Dounce Homogenization - Buffer D	Cell lysis by Dounce homogenization	HEPES-KOH pH 7.9 -KCl - Glycerol - EDTA - DTT - PMSF
Drying Solution	Drying SDS-PAGE gels	Ethanol - Glycerol
E. coli DNA Polymerase I Buffer, 10X
Earle's Balanced Salt Solution (EBSS)
Earle's Balanced Salt Solution - Bicarbonate-Free, 10X
EB Extraction Buffer	Purification of high molecular weight DNA from plant cells	Tris-Cl pH 8.0 - EDTA - NaCl - β-mercaptoethanol
EBC Lysis Buffer	Coimmunoprecipitation	Tris-Cl pH 8.0 - NaCl - IGEPAL CA-630 - Leupeptin - Aprotinin - PMSF - Sodium Orthovanadate - NaF
EDTA Glycogen Solution, 20X
Egg Buffer (C. elegans)	Imaging of embryo development in C. elegans	NaCl - KCl - CaCl₂ - MgCl₂ - HEPES pH 7.2
Egg Salts Solution	LIve imaging of early embryos from mouse, chick, Xenopus and C. elegans	NaCl - KCl - MgCl₂ - CaCl₂ - HEPES pH 7.4
Egg Water (ZFIN)

Nectagen

Solutions

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