SOB Medium


Rich medium for E. coli

Tryptone - Yeast Extract - NaCl - KCl - MgCl2

Composition 

Concentration Ingredient
20 g/l

Tryptone - Bacto
5 g/l

Yeast Extract - Bacto
0.5 g/l

NaCl
2.5 mM

KCl
10 mM

MgCl2

Note 

Add KCl after other ingredients have dissolved. Adjust pH to 7.0 with approximately 0.2 ml 5 M NaOH per liter. Autoclave. Add sterile 5 ml 2 M MgCl2 per liter just before use.

Calculator 

1000
Amount to add Ingredient Special Instructions
20.00 g Tryptone - Bacto (solid)
5.00 g Yeast Extract - Bacto (solid)
500.0 mg Sodium Chloride (solid)
2.5 ml Potassium Chloride, 1 M Add after Tryptone, Yeast Extract and NaCl have dissolved. Adjust pH to 7.0 with approximately 0.2 ml 5 M NaOH per liter and autoclave
5.0 ml Magnesium Chloride, 2 M Add sterile MgCl2 just before use


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 136