SNET


Purification of high molecular weight DNA by spooling

Tris-Cl pH 8.0 - EDTA - NaCl - SDS

Composition 

Concentration Ingredient
20 mM

Tris-Cl pH 8.0
5 mM

EDTA pH 8.0
400 mM

NaCl
1 % (wt:vol)

SDS

Calculator 

50
Amount to add Ingredient
1000.0 ul Tris-Cl pH 8.0, 1 M
500.0 ul EDTA pH 8.0, 0.5 M
4.0 ml Sodium Chloride, 5 M
5.0 ml SDS, 10% (wt:vol)
Storage
Temperature
  • Room Temperature


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 4