SM


Storage and dilution of bacteriophage λ stocks

NaCl - MgSO4 - Tris-Cl pH 7.5 - Gelatin

Composition 

Concentration Ingredient
5.8 g/l

NaCl
2 g/l

MgSO4

Heptahydrate
50 mM

Tris-Cl pH 7.5
0.01 % (wt:vol)

Gelatin - Bovine Skin Type B

Calculator 

500
Amount to add Ingredient
2.90 g Sodium Chloride (solid)
1.00 g Magnesium Sulfate Heptahydrate (solid)
25.0 ml Tris-Cl pH 7.5, 1 M
2.5 ml Gelatin - Bovine Skin Type B, 2% (wt:vol)


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 136