Slice Solution


Ballistic delivery of dyes to neurons

NaCl - KCl - NaH2PO4 - MgCl2 - CaCl2 - Glucose - NaH2CO3

Composition 

Concentration Ingredient
126 mM

NaCl
2.5 mM

KCl
1.25 mM

NaH2PO4
2 mM

MgCl2
2 mM

CaCl2
10 mM

Glucose
26 mM

NaHCO3

Note 

Gas with 5% CO2 - 95% O2 at room temperature before use. 

Calculator 

1000
Amount to add Ingredient
25.2 ml Sodium Chloride, 5 M
2.5 ml Potassium Chloride, 1 M
1250.0 ul Sodium Phosphate Monobasic, 1 M
2000.0 ul Magnesium Chloride, 1 M
2000.0 ul Calcium Chloride, 1 M
5.0 ml Glucose, 2 M
26.0 ml Sodium Bicarbonate, 1 M


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 49