Shoot-Inducing Medium | Nectagen

Shoot-Inducing Medium

Genetic manipulation of Arabidopsis

Gamborg's B5 Salts - Glucose - MES pH 5.7 - Agar - 2-iP - IAA - Kanamycin - Carbenicillin

Composition

Concentration	Ingredient	Details
1 X	Gamborg's B-5 Basal Salt Mixture
20 g/l	Glucose
0.5 g/l	MES Monohydrate
7.5 g/l	Agar - Plant Cell Culture	Plant tissue culture grade
5 ug/ml	2-iP
100 ng/ml	IAA Sodium Salt
50 ug/ml	Kanamycin Sulfate
500 ug/ml	Carbenicillin - Aqueous Solution

Note

Adjust pH to 5.7 with 1 N KOH

Calculator

Amount to add	Ingredient	Special Instructions
1000 ml	Gamborg's B-5 Basal Salt Mixture, 1X
20.00 g	Glucose (solid)
500.0 mg	MES Monohydrate (solid)	Adjust pH before addition of agar
7.50 g	Agar - Plant Cell Culture (solid)
5.0 ml	N6-(2-Isopentenyl)adenine, 5 mg/ml	Add after autoclaving other ingredients and cooling to ~60°C
100.0 ul	Indole-3-acetic Acid Sodium Salt, 1 mg/ml	Add after autoclaving other ingredients and cooling to ~60°C
1000.0 ul	Kanamycin Sulfate, 50 mg/ml	Add after autoclaving other ingredients and cooling to ~60°C
10.0 ml	Carbenicillin - Aqueous Solution, 50 mg/ml	Add after autoclaving other ingredients and cooling to ~60°C

Reference(s)

Reference	Title	PMID/ISBN	Note
Mellick and Rodgers (eds.) 2006	Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench	9780879698157	p. 97