Semi-Dry Transfer Buffer, 25X without Methanol


Semi-dry transfer of proteins to membranes for Western blottting

Tris Base - Glycine - SDS

Composition 

Concentration Ingredient
1.2 M

Tris Base
0.975 M

Glycine
0.925 % (wt:vol)

SDS

Note 

This recipe corrects rounding errors in the original.

Calculator 

400
Amount to add Ingredient
58.15 g Tris Base (solid)
29.28 g Glycine (solid)
3.70 g SDS (solid)


Reference(s)
Reference Title PMID/ISBN Note
Greenfield (ed.) 2014 Antibodies, A Laboratory Manual, 2nd Edition 9781936113811 p. 502