Semi-Dry Transfer Buffer


Semi-dry transfer of proteins to membranes for Western blottting

Tris Base - Glycine - SDS - Methanol

Composition 

Concentration Ingredient
48 mM

Tris Base
39 mM

Glycine
0.037 % (wt:vol)

SDS
20 % (vol:vol)

Methanol

Calculator 

100
Amount to add Ingredient
4.8 ml Tris Base, 1 M
3.9 ml Glycine, 1 M
370.0 ul SDS, 10% (wt:vol)
20.0 ml Methanol, 100% (vol:vol)


Reference(s)
Reference Title PMID/ISBN Note
Greenfield (ed.) 2014 Antibodies, A Laboratory Manual, 2nd Edition 9781936113811 p. 502