Second-strand Buffer, 10X


Isolation and amplification of RNA from single cells and small amounts of tissue

Tris-Cl pH 7.4 - KCl - MgCl2 - Ammonium Sufate

Composition 

Concentration Ingredient
1 M

Tris Base
200 mM

KCl
100 mM

MgCl2
400 mM

(NH4)2SO4

Note 

Adjust pH to 7.4 with HCl

Calculator 

1000
Amount to add Ingredient
121.14 g Tris Base (solid)
200.0 ml Potassium Chloride, 1 M
100.0 ml Magnesium Chloride, 1 M
100.0 ml Ammonium Sulfate, 4 M
Storage
Temperature
  • -20°C


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 13