SDS/Phosphate Sample Buffer


Alternative buffer system for discontinuous polyacrylamide gel electrophoresis

Sodium Phosphate pH 7.2 - SDS - Glycerol - Bromophenol Blue - DTT (optional)

Composition 

Concentration Ingredient Details
100 mM

Sodium Phosphate Buffer pH 7.2
1 % (wt:vol)

SDS
10 % (vol:vol)

Glycerol
0.001 % (wt:vol)

Bromophenol Blue
100 mM

DTT

 Optional

Calculator 

1000
Amount to add Ingredient Special Instructions
100.0 ml Sodium Phosphate Buffer pH 7.2, 1 M
100.0 ml SDS, 10% (wt:vol)
200.0 ml Glycerol, 50% (vol:vol)
1000.0 ul Bromophenol Blue, 1% (wt:vol)
100.0 ml DTT, 1 M Optional


Reference(s)
Reference Title PMID/ISBN Note
Greenfield (ed.) 2014 Antibodies, A Laboratory Manual, 2nd Edition 9781936113811 p. 759