SDS PAGE Stacking Gel


Protein electrophoresis

Acrylamide (29:1) - Tris-Cl pH 6.8 - SDS - APS - TEMED

Composition 

Concentration Ingredient
5 % (wt:vol)

Acrylamide 29:1
125 mM

Tris-Cl pH 6.8
0.1 % (wt:vol)

SDS
0.1 % (wt:vol)

APS
0.1 % (vol:vol)

TEMED

Calculator 

4000
Amount to add Ingredient
666.7 ul Acrylamide 29:1, 30% (wt:vol)
500.0 ul Tris-Cl pH 6.8, 1 M
40.0 ul SDS, 10% (wt:vol)
40.0 ul Ammonium Persulfate, 10% (wt:vol)
4.0 ul TEMED, 100% (vol:vol)
Storage
Estimated Shelf Life
  • None (Use fresh)


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 74