SDS PAGE Resolving Gel, 15% Acrylamide


Protein electrophoresis

Acrylamide (29:1) - Tris-Cl pH 8.8 - SDS - APS - TEMED

Composition 

Concentration Ingredient
15 % (wt:vol)

Acrylamide 29:1

375 mM

Tris-Cl pH 8.8

0.1 % (wt:vol)

SDS

0.1 % (wt:vol)

APS

0.08 % (vol:vol)

TEMED

Note 

Efficient separation of denatured proteins from 12 to 43 kD

Calculator 

10
Storage
Estimated Shelf Life
  • None (Use fresh)


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 76