SDS PAGE Resolving Gel, 10% Acrylamide


Protein electrophoresis

Acrylamide (29:1) - Tris-Cl pH 8.8 - SDS - APS - TEMED

Composition 

Concentration Ingredient
10 % (wt:vol)

Acrylamide 29:1
375 mM

Tris-Cl pH 8.8
0.1 % (wt:vol)

SDS
0.1 % (wt:vol)

APS
0.08 % (vol:vol)

TEMED

Note 

Efficient separation of denatured proteins from 16 to 68 kD

Calculator 

10
Amount to add Ingredient
3.3 ml Acrylamide 29:1, 30% (wt:vol)
2.5 ml Tris-Cl pH 8.8, 1.5 M
100.0 ul SDS, 10% (wt:vol)
100.0 ul Ammonium Persulfate, 10% (wt:vol)
8.0 ul TEMED, 100% (vol:vol)
Storage
Estimated Shelf Life
  • None (Use fresh)


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 pg. 75