SDS-Equilibration Buffer


Identification of novel protein kinase substrates

Tris-Cl pH 8.8 - Urea - Glycerol - SDS - Bromophenol Blue - DTT

Composition 

Concentration Ingredient
50 mM

Tris-Cl pH 8.8
6 M

Urea
30 % (vol:vol)

Glycerol
2 % (wt:vol)

SDS
0.002 % (wt:vol)

Bromophenol Blue
10 mg/ml

DTT

Calculator 

100
Amount to add Ingredient Special Instructions
5.0 ml Tris-Cl pH 8.8, 1 M
36.04 g Urea (solid)
30.0 ml Glycerol, 100% (vol:vol)
10.0 ml SDS, 20% (wt:vol)
200.0 ul Bromophenol Blue, 1% (wt:vol)
1.00 g DTT (solid) Add just before use


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 83