SDS Buffer - Chemical Cross-linking


Chemical chrosslinking with MBS 

Tris pH 6.8 - glycerol - β-mercaptoethanol - SDS - Coomassie Blue

Composition 

Concentration Ingredient Details
125 mM

Tris-Cl pH 6.8
20 % (vol:vol)

Glycerol
5 % (vol:vol)

β-mercaptoethanol
4 % (wt:vol)

SDS
0.003 % (vol:vol)

Coomassie Brilliant Blue G-250

 Recipe ambiguously specified "Coomassie blue (Sigma-Aldrich)"

Calculator 

10
Amount to add Ingredient
1250.0 ul Tris-Cl pH 6.8, 1 M
2000.0 ul Glycerol, 100% (vol:vol)
500.0 ul β-Mercaptoethanol, 100% (vol:vol)
2000.0 ul SDS, 20% (wt:vol)
0.0 mg Coomassie Brilliant Blue G-250 (solid)


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 76