Sarkosyl Buffer


Sarkosyl preparation of antigens from bacterial inclusion bodies

Tris-Cl pH 7.4 - NaCl - ZnCl2 - β-mercaptoethanol - Sarkosyl

Composition 

Concentration Ingredient
50 mM

Tris-Cl pH 7.4
300 mM

NaCl
5 mM

ZnCl2
10 mM

β-mercaptoethanol
10 % (wt:vol)

Sarkosyl

Calculator 

1
Amount to add Ingredient
50.0 ul Tris-Cl pH 7.4, 1 M
60.0 ul Sodium Chloride, 5 M
5.0 ul Zinc Chloride, 1 M
0.7 ul β-Mercaptoethanol, 14.4 M
500.0 ul Sodium Lauroyl Sarcosinate, 20% (wt:vol)


Reference(s)
Reference Title PMID/ISBN Note
Greenfield (ed.) 2014 Antibodies, A Laboratory Manual, 2nd Edition 9781936113811 p. 96