Sample Buffer


Two-dimensional isoelectric focusing/SDS-polyacrylamide gel electrophoresis

Urea - IGEPAL CA-630 - β-mercaptoethanol

Composition 

Concentration Ingredient
9 M

Urea

4 % (vol:vol)

IGEPAL CA-630

2 % (vol:vol)

β-mercaptoethanol

Calculator 

100
Amount to add Ingredient
54.05 g Urea (solid)
4.0 ml IGEPAL CA-630, 100% (vol:vol)
2000.0 ul β-Mercaptoethanol, 100% (vol:vol)


Reference(s)
Reference Title PMID/ISBN Note
Greenfield (ed.) 2014 Antibodies, A Laboratory Manual, 2nd Edition 9781936113811 p. 762