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Two-dimensional isoelectric focusing/SDS-polyacrylamide gel electrophoresis
Urea - IGEPAL CA-630 - β-mercaptoethanol
Concentration | Ingredient |
---|---|
9 M |
Urea |
4 % (vol:vol) |
IGEPAL CA-630 |
2 % (vol:vol) |
β-mercaptoethanol |
Amount to add | Ingredient |
---|---|
54.05 g | Urea (solid) |
4.0 ml | IGEPAL CA-630, 100% (vol:vol) |
2000.0 ul | β-Mercaptoethanol, 100% (vol:vol) |
Reference | Title | PMID/ISBN | Note |
---|---|---|---|
Greenfield (ed.) 2014 | Antibodies, A Laboratory Manual, 2nd Edition | 9781936113811 | p. 762 |