SAM, 30 mM


Methylation

S-(5′-Adenosyl)-L-methionine

Composition 

Concentration Ingredient
4.5 mM

H2SO4
10 % (vol:vol)

Ethanol
30 mM

SAM Iodide

Note 

Store as 100 ul aliquots, discard aliquot after use.  Half-life in solution at room temperature is approximately 30 minutes. 

Calculator 

1000
Amount to add Ingredient
4.5 ul Sulfuric Acid, 1 M
100.0 ul Ethanol, 100% (vol:vol)
15.8 mg S-(5′-Adenosyl)-L-methionine Iodide (solid)
Storage
Temperature
  • -20°C
Estimated Shelf Life
  • 12 months


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 40