Saline/EDTA Buffer Plus Glucose


Isolation of germ cells and tissue culture

EDTA - NaCl - KCl - Na2HPO4 - KH2PO4 - Phenol Red - Glucose

 

Composition 

Concentration Ingredient
0.2 g/l

EDTA
8 g/l

NaCl
0.2 g/l

KCl
1.15 g/l

Na2HPO4
0.2 g/l

KH2PO4
0.01 g/l

Phenol Red
0.2 g/l

Glucose

Note 

Adjust pH to 7.2

Calculator 

100
Amount to add Ingredient
20.0 mg EDTA (solid)
800.0 mg Sodium Chloride (solid)
20.0 mg Potassium Chloride (solid)
115.0 mg Sodium Phosphate Dibasic Anhydrous (solid)
20.0 mg Potassium Phosphate Monobasic (solid)
1.0 mg Phenol Red (solid)
20.0 mg Glucose (solid)


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 111