RNase Buffer


RNA digestion with RNase

Tris-Cl pH 8.0 - NaCl - DTT

Composition 

Concentration Ingredient
500 mM

NaCl
10 mM

Tris-Cl pH 8.0
10 mM

DTT

Calculator 

100
Amount to add Ingredient
500.000 g Sodium Chloride, 5 M
10.000 g Tris-Cl pH 8.0, 1 M
10.000 g DTT, 1 M


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 40