RNA Gel-loading Buffer


Nuclease S1 RNA protection

Formamide - Bromophenol Blue - Xylene Cyanol FF - EDTA - SDS

Composition 

Concentration Ingredient
95 % (vol:vol)

Formamide
1 mM

EDTA pH 8.0
0.025 % (wt:vol)

Bromophenol Blue
0.025 % (wt:vol)

Xylene Cyanol FF
5 mM

EDTA pH 8.0
0.025 % (wt:vol)

SDS

Note 

Add nuclease S1 to a concentration of 500 u/ml before use

Calculator 

10
Amount to add Ingredient
9.5 ml Formamide, 100% (vol:vol)
20.0 ul EDTA pH 8.0, 0.5 M
250.0 ul Bromophenol Blue, 1% (wt:vol)
250.0 ul Xylene Cyanol FF, 1% (wt:vol)
100.0 ul EDTA pH 8.0, 0.5 M
12.5 ul SDS, 20% (wt:vol)
Storage
Temperature
  • -20°C
Precaution(s)
  • Under inert gas


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 25