RNA Extraction Buffer - Vanacyl-Ribonucleoside Complexes | Nectagen

RNA Extraction Buffer - Vanacyl-Ribonucleoside Complexes

RNA purification from mammalian cells

NaCl - MgCl₂ - Tris-Cl pH 8.6 - IGEPAL CA-630 - DTT - Vanadyl-Ribonucleoside Complexes

Composition

Concentration	Ingredient	Details
140 mM	NaCl
1.5 mM	MgCl₂
10 mM	Tris-Cl pH 8.6
0.5 % (vol:vol)	IGEPAL® CA-630	Substitute for NP-40
1 mM	DTT
20 mM	Ribonucleoside Vanadyl Complexes

Calculator

Amount to add	Ingredient
28.0 ml	Sodium Chloride, 5 M
1500.0 ul	Magnesium Chloride, 1 M
10.0 ml	Tris-Cl pH 8.6, 1 M
50.0 ml	IGEPAL CA-630, 10% (vol:vol)
1000.0 ul	DTT, 1 M
100.0 ml	Ribonucleoside Vanadyl Complexes, 200 mM

Reference(s)

Reference	Title	PMID/ISBN	Note
Roskams and Rodgers (eds.) 2002	Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench	9780879696306	p. 51