RNA Extraction Buffer - RNase Inhibitor | Nectagen

RNA Extraction Buffer - RNase Inhibitor

RNA purification from mammalian cells

NaCl - MgCl2 - Tris-Cl pH 8.6 - IGEPAL CA-630 - DTT - RNase Inhibitor

Composition

Concentration	Ingredient	Details
140 mM	NaCl
1.5 mM	MgCl₂
10 mM	Tris-Cl pH 8.6
0.5 % (vol:vol)	IGEPAL® CA-630	Substitute for NP-40
1 mM	DTT
1000 units/ml	Ribonuclease Inhibitor

Calculator

Amount to add	Ingredient
280.0 ul	Sodium Chloride, 5 M
15.0 ul	Magnesium Chloride, 1 M
100.0 ul	Tris-Cl pH 8.6, 1 M
500.0 ul	IGEPAL CA-630, 10% (vol:vol)
10.0 ul	DTT, 1 M
500.0 ul	Ribonuclease Inhibitor, 20,000 units/ml

Reference(s)

Reference	Title	PMID/ISBN	Note
Roskams and Rodgers (eds.) 2002	Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench	9780879696306	p. 51