RNA Amplification Buffer, 10X


Isolation and amplification of RNA from single cells and small amounts of tissue

Tris-Cl pH 7.5 -  MgCl2 - NaCl - Spermidine

Composition 

Concentration Ingredient
400 mM

Tris-Cl pH 7.5
70 mM

MgCl2
100 mM

NaCl
20 mM

Spermidine

Calculator 

1000
Amount to add Ingredient Special Instructions
400.0 ml Tris-Cl pH 7.5, 1 M
70.0 ml Magnesium Chloride, 1 M
20.0 ml Sodium Chloride, 5 M
200.0 ml Spermidine, 100 mM Filter before adding spermidine


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 13