RIPA Buffer


Radioimmunoprecipitation assay buffer

Composition 

Concentration Ingredient
150 mM

NaCl
25 mM

Tris-Cl pH 8.0
1 % (vol:vol)

Triton™ X-100
0.5 % (wt:vol)

Sodium Deoxycholate
0.1 % (wt:vol)

SDS

Calculator 

250
Amount to add Ingredient
7.5 ml Sodium Chloride, 5 M
6.3 ml Tris-Cl pH 8.0, 1 M
25.0 ml Triton™ X-100, 10% (vol:vol)
12.5 ml Sodium Deoxycholate, 10% (wt:vol)
2.5 ml SDS, 10% (wt:vol)