RIPA Buffer - Chromatin Immunoprecipitation


Chromatin immunoprecipitation

Tris-Cl pH 8.0 - EDTA - EGTA - Triton X-100 - Sodium Deoxycholate - SDS - NaCl - PMSF

Composition 

Concentration Ingredient
10 mM

Tris-Cl pH 8.0
1 mM

EDTA pH 8.0
0.5 mM

EGTA pH 7.0
140 mM

NaCl
1 % (vol:vol)

Triton™ X-100
0.1 % (wt:vol)

Sodium Deoxycholate
0.1 % (wt:vol)

SDS
1 mM

PMSF

Calculator 

100
Amount to add Ingredient Special Instructions
1000.0 ul Tris-Cl pH 8.0, 1 M
200.0 ul EDTA pH 8.0, 0.5 M
50.0 ul EGTA pH 7.0, 1 M
2.8 ml Sodium Chloride, 5 M
10.0 ml Triton™ X-100, 10% (vol:vol)
1000.0 ul Sodium Deoxycholate, 10% (wt:vol)
1000.0 ul SDS, 10% (wt:vol)
1000.0 ul PMSF, 100 mM Add just before use


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 73