RIPA Buffer


Chromatin immunprecipitation

HEPES-KOH pH 7.6 - LiCl - EDTA - IGEPAL CA-630 - Sodium Deoxycholate

Composition 

Concentration Ingredient
50 mM

HEPES-KOH pH 7.6
500 mM

LiCl
1 mM

EDTA pH 8.0
1 % (vol:vol)

IGEPAL® CA-630
0.7 % (wt:vol)

Sodium Deoxycholate

Calculator 

15
Amount to add Ingredient
750.0 ul HEPES-KOH pH 7.6, 1 M
937.5 ul Lithium Chloride, 8 M
30.0 ul EDTA pH 8.0, 0.5 M
1500.0 ul IGEPAL CA-630, 10% (vol:vol)
1050.0 ul Sodium Deoxycholate, 10% (wt:vol)


Reference(s)
Reference Title PMID/ISBN Note
Greenfield (ed.) 2014 Antibodies, A Laboratory Manual, 2nd Edition 9781936113811 p. 624