Reswelling Buffer


Two-dimensional gel electrophoresis

Urea - DTT - CHAP -Pharmalyte

Composition 

Concentration Ingredient
8 M

Urea
1 % (wt:vol)

CHAPS
0.4 % (wt:vol)

DTT
2.5 % (vol:vol)

Pharmalyte pH 3.0-10.0

Note 

Dissolve urea in 90% of final volume.  Add Serdolit MB-1 mixed-bed ion exchange resin to 1% (wt:vol).  Incubate for 10 min. with stirring and filter.  Add remaining ingredients. 

Calculator 

50
Amount to add Ingredient
24.02 g Urea (solid)
500.0 mg CHAPS (solid)
200.0 mg DTT (solid)
1250.0 ul Pharmalyte pH 3.0-10.0, 100% (vol:vol)
Storage
Estimated Shelf Life
  • None (Use fresh)


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 62