Rescue Buffer


Two-hybrid systems

Tris-Cl pH 7.5 - EDTA - β-mercaptoethanol

Composition 

Concentration Ingredient
50 mM

Tris-Cl pH 7.5
10 mM

EDTA pH 8.0
0.3 % (vol:vol)

β-mercaptoethanol

Calculator 

1000
Amount to add Ingredient
50.0 ml Tris-Cl pH 7.5, 1 M
20.0 ml EDTA pH 8.0, 0.5 M
3.0 ml β-Mercaptoethanol, 100% (vol:vol)
Storage
Estimated Shelf Life
  • None (Use fresh)


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 78