Renilla Luciferase Assay Buffer


Protein fragment complementation assays

KCl - NaCl - HEPES pH 7.4 - DTT - MgCl2 - EDTA - IGEPAL CA-630

Composition 

Concentration Ingredient Details
50 mM

KCl

50 mM

NaCl

20 mM

HEPES pH 8.0

2.5 mM

MgCl2

2 mM

EDTA pH 8.0

0.2 % (vol:vol)

IGEPAL® CA-630

Substitute for NP-40
5 mM

DTT

Calculator 

100
Amount to add Ingredient
5.0 ml Potassium Chloride, 1 M
1000.0 ul Sodium Chloride, 5 M
2000.0 ul HEPES pH 8.0, 1 M
250.0 ul Magnesium Chloride, 1 M
400.0 ul EDTA pH 8.0, 0.5 M
2000.0 ul IGEPAL CA-630, 10% (vol:vol)
500.0 ul DTT, 1 M


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 88