Refolding Buffer - IMAC


Immobilized metal-ion affinity chromatography

Imidazole - NaCl - βME - Tris

Composition 

Concentration Ingredient
20 mM

Imidazole
0.5 M

NaCl
1 mM

β-mercaptoethanol
20 mM

Tris-Cl pH 8.0

Calculator 

100
Amount to add Ingredient
2000.0 ul Imidazole, 1 M
10.0 ml Sodium Chloride, 5 M
6.9 ul β-Mercaptoethanol, 14.4 M
2000.0 ul Tris-Cl pH 8.0, 1 M


Reference(s)
Reference Title PMID/ISBN
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157