Rearing Solution


Rearing Xenopus

NaCl - KCl - CaNO3 - MgSO4 - HEPES pH 7.4 

Composition 

Concentration Ingredient
58.2 mM

NaCl
0.67 mM

KCl
0.34 mM

Ca(NO3)2
0.78 mM

MgSO4
50 mM

HEPES pH 7.4

Calculator 

1000
Amount to add Ingredient
11.6 ml Sodium Chloride, 5 M
670.0 ul Potassium Chloride, 1 M
680.0 ul Calcium Nitrate, 0.5 M
780.0 ul Magnesium Sulfate, 1 M
50.0 ml HEPES pH 7.4, 1 M


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 41