Proteinase Digestion Buffer


In situ peptide mapping

Tris-Cl pH 6.8 - SDS - Glycerol - Bromophenol Blue

Composition 

Concentration Ingredient
0.125 M

Tris-Cl pH 6.8
0.1 % (wt:vol)

SDS
20 % (vol:vol)

Glycerol
0.005 % (wt:vol)

Bromophenol Blue

Calculator 

1000
Amount to add Ingredient
125.0 ml Tris-Cl pH 6.8, 1 M
10.0 ml SDS, 10% (wt:vol)
200.0 ml Glycerol, 100% (vol:vol)
5.0 ml Bromophenol Blue, 1% (wt:vol)


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 68