Proteinase Digestion Buffer


RNA purification from mammalian cells

Tris-Cl pH 8.0 - EDTA - NaCl - SDS

Composition 

Concentration Ingredient
200 mM

Tris-Cl pH 8.0
25 mM

EDTA pH 8.0
300 mM

NaCl
2 % (wt:vol)

SDS

Calculator 

1000
Amount to add Ingredient
200.0 ml Tris-Cl pH 8.0, 1 M
50.0 ml EDTA pH 8.0, 0.5 M
60.0 ml Sodium Chloride, 5 M
200.0 ml SDS, 10% (wt:vol)


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 51