Protein Transfer Buffer


Western blotting

Tris - Glycine - SDS - Methanol

Composition 

Concentration Ingredient
2.9 g/l

Glycine
5.8 g/l

Tris Base
0.37 g/l

SDS
20 % (vol:vol)

Methanol

Calculator 

1000
Amount to add Ingredient
2.90 g Glycine (solid)
5.80 g Tris Base (solid)
370.0 mg SDS (solid)
200.0 ml Methanol, 100% (vol:vol)


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 81