Prehybridization Solution


Southern blotting

SSC - Denhardt's - SDS - Denatured Salmon Sperm DNA

Composition 

Concentration Ingredient
6 X

SSC
10 X

Denhardt's Reagent
1 % (wt:vol)

SDS
250 ug/ml

DNA - Single Stranded Salmon Sperm

Calculator 

20
Amount to add Ingredient Special Instructions
6 ml SSC, 20 X
4 ml Denhardt's Reagent, 50X
2000.0 ul SDS, 10% (wt:vol)
500.0 ul DNA - Single Stranded Salmon Sperm, 10 mg/ml Add just before use after preheating solution to 65°C
Storage
Temperature
  • -20°C


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 89