Powdered Supplement Mixture for SC Medium


Component of synthetic media for S. cerevesiae

Adenine - Ala - Arg - Asn - Asp - Cys - Gln - Glu - Gly - His - Inositol - Ile - Leu - Lys - Met - PABA - Phe - Pro - Ser - Thr - Trp - Tyr - Ura - Val

Composition 

Concentration Ingredient
1.071 % (wt:wt)

Adenine Hemisulfate Salt
4.283 % (wt:wt)

L-Alanine
4.283 % (wt:wt)

L-Arginine HCl
4.283 % (wt:wt)

L-Asparagine
4.283 % (wt:wt)

L-Aspartic Acid
4.283 % (wt:wt)

L-Cysteine HCl
4.283 % (wt:wt)

L-Glutamine
4.283 % (wt:wt)

L-Glutamic Acid Monosodium Salt
4.283 % (wt:wt)

Sodium Glycinate
4.283 % (wt:wt)

L-Histidine Monohydrochloride
4.283 % (wt:wt)

Inositol (myo)
4.283 % (wt:wt)

L-Isoleucine
8.565 % (wt:wt)

L-Leucine
4.283 % (wt:wt)

L-Lysine HCl
4.283 % (wt:wt)

L-Methionine
0.428 % (wt:wt)

4-Aminobenzoic Acid
4.283 % (wt:wt)

L-Phenylalanine
4.283 % (wt:wt)

L-Proline
4.283 % (wt:wt)

L-Serine
4.283 % (wt:wt)

L-Threonine
4.283 % (wt:wt)

L-Tryptophan
4.283 % (wt:wt)

L-Tyrosine
4.283 % (wt:wt)

Uracil
4.283 % (wt:wt)

Valine

Note 

Setting desired amount to 467 g yields round numbers for weighing (0.2 g, 0.5 g, 2 g, 4g).  Before each use, shake the mixture in a plastic bottle with clean glass marbles

Calculator 

467
Amount to add Ingredient
5.002 g Adenine Hemisulfate Salt (solid)
20.002 g L-Alanine (solid)
20.002 g L-Arginine Monohydrochloride (solid)
20.002 g L-Asparagine Monohydrate (solid)
20.002 g L-Aspartic Acid (solid)
20.002 g L-Cysteine Hydrochloride Monohydrate (solid)
20.002 g L-Glutamine (solid)
20.002 g L-Glutamic Acid Monosodium Salt (solid)
20.002 g Sodium Glycinate
20.002 g L-Histidine Monohydrochloride Monohydrate (solid)
20.002 g Inositol (myo) (solid)
20.002 g L-Isoleucine (solid)
39.999 g L-Leucine (solid)
20.002 g L-Lysine Monohydrochloride (solid)
20.002 g L-Methionine (solid)
1.999 g 4-Aminobenzoic Acid (solid)
20.002 g L-Phenylalanine (solid)
20.002 g L-Proline (solid)
20.002 g L-Serine (solid)
20.002 g L-Threonine (solid)
20.002 g L-Tryptophan (solid)
20.002 g L-Tyrosine (solid)
20.002 g Uracil (solid)
20.002 g L-Valine (solid)
Storage
Precaution(s)
  • Under inert gas


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 144