PNK Buffer, 10X


Buffer for phosphorylation of DNA with polynucleotide kinase

Tris-Cl pH 7.6 - MgCl2 - DTT

Composition 

Concentration Ingredient
700 mM

Tris-Cl pH 7.6
100 mM

MgCl2
50 mM

DTT

Calculator 

1
Amount to add Ingredient
700.0 ul Tris-Cl pH 7.6, 1 M
100.0 ul Magnesium Chloride, 1 M
50.0 ul DTT, 1 M
Storage
Temperature
  • -20°C
Estimated Shelf Life
  • 6 months


Reference(s)
Reference Title PMID/ISBN Note
Gallagher and Wiley (eds.) 2012 Current Protocols Essential Laboratory Techniques 978-0470942413 p. 8.4.20