PK Buffer, 2X | Nectagen

PK Buffer, 2X

Use and labeling of GST fusions

Potassium Phosphate pH 7.15 - MgCl₂ - NaF - DTT

Composition

Concentration	Ingredient
100 mM	Potassium Phosphate Buffer pH 7.15
20 mM	MgCl₂
10 mM	NaF
9 mM	DTT

Calculator

Amount to add	Ingredient
100.0 ml	Potassium Phosphate Buffer pH 7.15, 1 M
20.0 ml	Magnesium Chloride, 1 M
10.0 ml	Sodium Fluoride, 1 M
9.0 ml	DTT, 1 M

Reference(s)

Reference	Title	PMID/ISBN	Note
Mellick and Rodgers (eds.) 2006	Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench	9780879698157	p. 75