PK Buffer, 2X


RNAi in Drosophila

Tris-Cl pH 7.5 - EDTA - NaCl - SDS

Composition 

Concentration Ingredient
200 mM

Tris-Cl pH 7.5
25 mM

EDTA pH 8.0
300 mM

NaCl
2 % (wt:vol)

SDS

Calculator 

1000
Amount to add Ingredient
200.0 ml Tris-Cl pH 7.5, 1 M
50.0 ml EDTA pH 8.0, 0.5 M
60.0 ml Sodium Chloride, 5 M
200.0 ml SDS, 10% (wt:vol)


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 31