Pipette Solution


Ballistic delivery of dyes to neurons

Potassium Gluconate - KCl - MgCl2 - HEPES - EGTA - CaCl2 - ATP - GTP - Glucose

Composition 

Concentration Ingredient
120 mM

Potassium Gluconate
10 mM

KCl
1 mM

MgCl2
10 mM

HEPES
0.1 mM

EGTA pH 7.0
25 uM

CaCl2
1 mM

ATP
0.2 mM

GTP
4 mM

Glucose

Note 

Adjust pH to 7.2 with KOH

Calculator 

10
Amount to add Ingredient
600.0 ul Potassium Gluconate, 2 M
100.0 ul Potassium Chloride, 1 M
10.0 ul Magnesium Chloride, 1 M
100.0 ul HEPES, 1 M
1.0 ul EGTA pH 7.0, 1 M
0.3 ul Calcium Chloride, 1 M
100.0 ul ATP, 100 mM
20.0 ul GTP, 100 mM
20.0 ul Glucose, 2 M


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 50