Phosphate-Buffer Stock Solution for Yeast X-gal Plates, 10X


Component of indicator media for lacZ+/- S. cerevisiae

KH2PO4 - Ammonium Sulfate - KOH

Composition 

Concentration Ingredient
1 M

KH2PO4
0.15 M

(NH4)2SO4
0.75 M

KOH

Calculator 

1000
Amount to add Ingredient
136.09 g Potassium Phosphate Monobasic (solid)
75.0 ml Ammonium Sulfate, 2 M
42.08 g Potassium Hydroxide (solid)


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 149